摘要
用Trizol法抽提出中华绒螯蟹眼柄视上神经节的总RNA和肌肉的总RNA,紫外分光光度计检测2种总RNA的纯度,1.0%琼脂糖凝胶电泳检测分析RNA的质量。实验结果表明,眼柄视上神经节和肌肉的总RNA纯度均较高,完整性较好,RNA基本无降解,总RNA的含量和纯度均可以用于后续的抑制消减杂交实验,为构建中华绒螯蟹眼柄视上神经节特异性表达的cDNA文库打下了良好的基础。
In this experimentation, we distilled total RNA in eyestalk and muscle in Eriocheir sinensis, mensurated the pureness of RNA with spectrophotometer, measured and analysed mass of RNA with 1.0% agarose gel, The experiment result proved that The total RNA in eyestalk and muscle were highly pure and integrity, almost not decompounded, and the both total RNA content can be used in the subsequent supression subtructive hybridization experimentation, providing favourable base for constructing the cDNA library of eyestalk speciality expression.
基金
河北省自然科学基金"中华绒螯蟹性腺抑制激素基因的克隆与表达(C2008000591)"
