摘要
目的探讨ATP结合盒转运子A1基因(ABCA1)R219K的多态性与心房颤动(房颤)相关性。方法选择250例房颤患者(房颤组)和250例非房颤者(对照组)为研究对象。采用聚合酶链式反应-限制性片段长度多态性测定ABCA1基因的多态性,并检测所有研究对象的C反应蛋白(CRP)和高密度脂蛋白胆固醇(HDL-C)血清浓度;比较两组不同基因型、不同等位基因的分布及CRP、HDL-C的血清浓度。结果房颤组与对照组ABCA1R219KRR型、RK型、KK型基因型频率分别为42.0%、42.8%、15.2%和34.0%、43.2%、22.8%,房颤组KK型明显低于对照组(P=0.03);房颤组与对照组R等位基因、K等位基因分布频率为63.4%、36.6%和55.6%、44.4%,K等位基因分布频率在房颤组中较对照组明显降低(P=0.012);房颤组CRP浓度明显高于对照组(P=0.004),HDL-C浓度无明显差异;在不同基因型之间CRP、HDL-C浓度比较中发现,RR基因型CRP浓度显著高于KK型(P=0.013);而HDL-C浓度RR基因型低于RK型和KK型(P分别为0.009、0.027)。结论房颤的炎性指标CRP显著升高;ABCA1R219K位点K等位基因可能是房颤发生的一种保护因素。
Objective To investigate the association of ATP binding cassette transporter A1 （ABCA1） gene R219K polymorphisms with atrial fibrillation （AF） in Chinese population. Methods A total of 250 patients with AF and 250 control subjects were selected. Polymerase chain reaction-restricted fragments length polymorphism （PCR-RFLP） was used to determine the ABCA1 genotype, and the serum concentration of C-reactive protein （CRP） and high-density lipoprotein cholesterol （HDL-C） were measured in all the subjects. Results The frequency of the RR, RK, KK, allele R, allele K genotype of ABCA1 in AF group and control group was 42.0 %, 42.8 %, 15.2 %, 34.0 %, 43.2 % and 22.8 %, 63.4%, 36.6%, 55.6 %, 44.4 %, respectively. The frequency of the KK genotype was significantly higher in the control group than in the case group （P=-0.03）, and the frequency of the allele K genotype was significantly different between the two groups （P=-0.012）. The serum CRP concentrations was significantly higher in AF group than in the control group （P=-0.004）, but serum HDL-C level showed no difference between the two groups. The serum CRP concentrations were significantly higher in patients with RR genotype than in those with KK genotype （P=-0.013）, and patients with RR genotype had significantly lower HDL-C level than those with RK and KK genotypes （P=0.009 and 0.027, respectively）. Conclusion Patients with AF have elevated serum CRP level in comparison with healthy individuals, and the K allele of R219K polymorphism is an independent protective factor against AF.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第3期494-496,499,共4页
Journal of Southern Medical University
基金
广东省科技计划项目(2008B030301142)
关键词
心房颤动
ATP结合盒转运子A1
基因多态性
C反应蛋白
atrial fibrillation
ATP-binding cassette transporter A1
gene polymorphisms
C-reactive protein
