摘要
目的：研究Cx26在肝细胞癌及正常肝组织中的表达情况。方法：免疫组化和RT-PCR检测Cx26和PCNA的表达。结果：①Cx26mRNA在正常肝组织组和肝癌组的表达水平分别为0．82±0．37和0．38±0．10；Cx26mRNA在HCC高分化组中的表达高于其在低分化组中的表达水平，分别为0．48±0．12和0．21±0．05。②Cx26在所有正常肝组织中均高表达，其在肝癌组织中的阳性率为64．71％，Cx26的表达随着肝癌分化程度的增加而增强。③在肝癌组织中，PCNA-LI在Cx26蛋白阳性表达组和阴性表达组分别为32．72％±4.21％和56．67％±7．21％，Cx26蛋白阳性者的PCNA-LI明显低于阴性者，PCNA表达水平与Cx26蛋白表达呈负相关。结论：①在肝癌组织中，Cx26的表达随着组织分化程度的降低而降低。②Cx26可作为评价肝癌预后的指标之一。
Objective： To study the expression of connexin 26 （Cx26） in hepatocellular carcinoma （HCC） and normal liver tissues. Methods： Immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR） were employed to determine the expression of Cx26 and proliferating cell nuclear antigen （PCNA）. Results： The expression of Cx26 mRNA in normal liver tissues and hepatocellular carcinoma was 0.82±0.37 and 0.38±0.10, respectively. The expression level of Cx26 mRNA in the well differentiated group （Grade Ⅰ-Ⅱ） was 0.48＋0.12, higher than the 0.21±0.05 found in the poorly differentiated group （Grade Ⅲ-Ⅳ）. Normal liver tissues were positive for Cx26 staining. The positive rate of Cx26 protein expression was 61.7% in the hepatocellular carcinoma samples. The expression of Cx26 protein decreased as the malignant degree increased. PCNA protein expression was 32.72±4.21% in the Cx26-positive group and 56.67±7.21% in the Cx26-negative group. The expression of PCNA protein was negatively correlated with the expression of Cx26 protein. Conclusion： The expression of Cx26 decreases as the differentiation degree of HCC increases. Cx26 can be used as a prognostic indicator for hepatocellular carcinoma.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2009年第5期272-274,278,共4页
Chinese Journal of Clinical Oncology
基金
山东省“泰山学者”建设工程专项经费(编号:2006GG3202016)
山东省科技攻关计划项目资助(编号:2006GG3202016)~~
关键词
连接蛋白-26
增殖细胞核抗原
肝细胞癌
Connexin-26
Proliferating cell nuclear antigen
Hepatocellular carcinoma
