摘要
依据传统形态学、生理生化特征及16S rRNA基因序列,将一株对十字花科根肿病有很好防治效果的菌株XF-1鉴定为枯草芽孢杆菌。采用细菌的通用引物P0,P6扩增16S rRNA基因片段,得到1513 bp的DNA片段,其序列与枯草芽孢杆菌的序列的同源性高达99%。与病原真菌对峙培养的结果表明,该菌株能对供试的卵菌、子囊菌、担子菌和半知菌的21个植物病原真菌均有很好的抑制作用。
A biocontrol strain, XF-1, was identified as Bacillus subtilis based on morphology, physiology, biochemistry and biotechnology, which could control clubroot of cruciferous crops effectively. The sequence of 16S rRNA gene has 99% identity to B. subtilis according to the amplified fragment of 1513 bp with a pair of universal primers, P0 and P6, of bacteria. On co-culture plates this strain showed high suppression effect on 21 fungal pathogens involved in oomycetes, ascomycetes, basidiomycetes and deuteromycetes.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第2期190-194,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省重点基金项目(2008CC024)
科技部国际科技合作项目(2008GR0999)
关键词
枯草芽孢杆菌XF-1
鉴定
十字花科根肿病
生物防治
Bacillus subtilis XF-1
identification
clubroot of cruciferous crops
biological control
