摘要
采用超临界CO2萃取技术联合气相色谱-质谱法检测了黄花蒿Artemisia annua萃取产物化学成分,并与水蒸气蒸馏法相比较。结果从超临界CO2萃取提取物成分中确定了86种化合物,主要成分为8,9-脱氢-9-甲酰环异长叶烯(18.22%);(all-Z)-5,8,11,14,17,二十碳五烯酸甲基酯(8.39%)等。从水蒸气蒸馏提取物成分中确定了62种化合物,主要成分为4-甲氧基-6-(2-丙烯基)-1,3-苯丙二氧杂环戊烯(24.28%);松油醇(17.47%)等。2种方法的共有成分32种,分别占鉴定积分面积的40.54%和79.49%。与传统水蒸气蒸馏法相比,采用超临界CO2法能更加真实、全面地反映黄花蒿的化学组分。
Essential oil from Artemisia annua was extracted by supercritical fluid CO2 extraction（SFE-CO2） and water steam distillation（WSD）.And the components were analyzed by GC—MS.The result indicated that 86 compounds were identified from the essential oil obtained by SFE-CO2 and the main components were 8,9-dehydro-9-formyl-cycloisolongifolene（18.22%）,（all-Z）-5,8,11,14,17-eicosapentaenoic acid methyl ester（8.39%）,etc;61 compounds were identified from the essential oil obtained by WSD and its main components were 1,3-Benzodioxole,4-methoxy-6-（2-propenyl）（24.28%）,2-Cyclohexen-1-ol（17.47%）,etc.There were 31 components were same in essential oil through two extraction methods,which accounted for 39.45% and 78.40% of the total integral area while identifying the essential oil.Compared with the traditional steam distillation method,the use of SFE-CO2 reflected more real and comprehensive chemical components of Artemisia annua.
出处
《草业科学》
CAS
CSCD
北大核心
2009年第3期57-63,共7页
Pratacultural Science
基金
教育部高等学校科技创新工程重大项目培育资金项目(706056)
关键词
黄花蒿
超临界CO2萃取
水蒸气蒸馏
Artemisia annua
CO2 supercritical fluid extraction
steam distillation
