摘要
测定和分析了野猪与大白猪杂交后F1代群体内8个与繁殖性状相关基因的群体遗传情况,以期为猪的杂交育种以及标记辅助选择提供一定的理论依据。采用PCR-RFLP法对248头杂交猪(大白♀×野猪♂)的ESR、FSHβ、GPX5、FUT1、PRLR、NCOA1、OPN和RBP4基因的多态位点进行了检测,并计算了每个位点的基因型频率和基因频率。大白猪群体内引入野猪血统后,F1代的ESR、FSHβ和PRLR的优势等位基因频率都有不同程度的下降,FUT1和GPX5的优势等位基因频率与现有研究结果基本一致,而NCOA1和OPN的优势基因频率都高于已检测的杂交品种,只有RBP4基因处于哈代-温伯格平衡状态。野猪与家猪杂交育种工作中,要选择ESR、FSHβ和PRLR的优势等位基因频率较高的母本群体。
To detect and analyze the group heredity of eight reproduction genes in F1 generation of wild boara nd Large White pig,hope the result could be useful for crossbreed and marker assisted selection（MAS）.Theg enotypic frequencies and allele frequencies of eight genes related to reproduction,i.e.ESR,FSHβ,GPX5,FUT1,PRLR,NCOA1,OPN and RBP4 were tested in the 248 F1 generation hybrid pig（Large White♀×wildb oar♂）by PCR-RFLP.In F1 crossbred,ESR,FSHβand PRLR predominance allele frequency had decreaset rend,except FUT1 and GPX5,but NCOA1 and OPN＇s were all higher than had been detected in crossbreds,o nly RBP4 was existed in Hardy-Weinberg equilibrium state.In breeding work of wild boar and domestic pig,i t should select female parent group which contained highly predominance allele frequency of ESR、FSHβandP RLR genes.
出处
《中国农学通报》
CSCD
北大核心
2009年第4期10-13,共4页
Chinese Agricultural Science Bulletin
基金
"十一五"国家科技支撑计划重点项目"畜禽特色优异基因资源挖掘与种质创新"(2008BADB2B01)。
关键词
野家杂交猪
繁殖性状
候选基因
PCR-RFLP
wild boar and domestic hybrid pig,reproduction trait,candidate gene,PCR-RFLP
