摘要
从一个水稻启动子捕获突变群体中筛选到一个T0及T1代GUS组织化学检测均表现胚乳特异表达的启动子捕获系w9101,其T0、T1代自交后代标记基因分离规律及Southern blot分析表明它是一个单T-DNA插入的启动子捕获系;通过TAIL-PCR获得其T-DNA插入的侧翼序列,并在NCBI上对其侧翼序列进行比对,发现该捕获系T-DNA插入位点位于一个推测的肽转运子基因(暂命名为Peptide transporter 9101,PTR9101)启动子内;w9101不同发育时期不同组织RT-PCR检测表明,PTR9101为胚乳特异性表达基因;生物信息学分析表明PTR9101蛋白整条肽链表现疏水性,其跨膜区域和CHL1(The Nitrate TransporterAtNRT1.1)一样有12个α-螺旋跨膜区,因此推测PTR9101是一个肽转运子基因。
A promoter trapping line （accession no. w9101）, in which the β-glucuronidase （GUS） reporter gene was endosperm-specific expression in both Toand T0 generations, was identified from a promoter trapping population in rice （Oryza sativa L. ssp. indica）. Genetic analysis and the Southern blot hybridization of the marker gene indicated that w9101 was a trapping line with a copy of T-DNA insertion. The flanking sequence of T-DNA insertion site was isolated by TAIL-PCR in w9101. The BLAST result of the sequence showed that the T-DNA insertion site was located in the promoter sequence of a putative peptide transporter gene in chromosome 6 of rice. It was tentatively named as PTR9101. RT-PCR analysis of the PTR9101 gene revealed that it was specifically expressed in rice endosperm. Further analysis by bioinformatics method indicated that the PTR9101 protein was hydrophobic with 12 α- helix membrane-spanning domain and similar to that of CHL1 （The Nitrate Transporter AtNRT1.1） in Arabidopsis. It was thus concluded that PTR9101 was a hydrophobic peptide transporter gene.
出处
《福建农业学报》
CAS
2009年第1期6-10,共5页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金(B0320002)
关键词
水稻
胚乳
启动子捕获系
肽转运子基因
Rice （Oryza sativa L. ）
endosperm
promoter trapping line
PTR9101
