摘要
[目的]探讨沙利度胺对人鼻咽癌细胞株细胞周期分布及体外放射敏感性的影响。[方法]应用MTT法检测不同时间点、不同浓度沙利度胺对人鼻咽癌细胞株CNE-2细胞增殖的抑制率。采用沙利度胺及放射线单独或联合作用于CNE-2细胞,用克隆形成法经单击多靶模型拟合细胞存活曲线,获得不同处理条件下的放射增敏比;流式细胞技术分析沙利度胺对CNE-2细胞周期分布的影响。[结果]沙利度胺可抑制CNE-2细胞的增殖,且呈浓度依赖性;20mg/L和40mg/L沙利度胺作用CNE-2细胞24h后均见到放射增敏作用,SER分别为1.29(0.941/0.728)和1.57(0.941/0.598);流式细胞仪分析表明沙利度胺作用24h,随着沙利度胺浓度的增加,CNE-2细胞中处于G0/G1期的细胞增多,S期和G2/M期细胞减少,但变化差异无显著性。[结论]沙利度胺能够抑制CNE-2细胞的增殖,并具有一定的放射增敏作用,为临床放疗和沙利度胺的联合应用提供了实验依据。
[Purpose ] To evaluate the effect of thalidomide on the cell cycle and the radiosensitivity in human nasopharyngeal carcinoma cell line CNE-2. [Methods] Cell growth inhibition was assessed by MTT（microculture tetrazolium assay） after incubated with thalidomide at various concentrations and times; CNE-2 cell was exposed to thalidomide or/and irradiation, the cell survival curves and SERs （sensitive enhancement ratio） were obtained with the colony formation assay; cell-cycle kinetics was analyzed by flow cytometry （FCM）. [Results] Growth of CNE-2 cell was in- hibited in a dose-dependent manner; thalidomide 20mg/L and 40mg/L both had radiosensitive effect on CNE-2 cell exposed for 24h, and SERs of which were 1.29 and 1.57; When CNE-2 cell was treated with different concentrations of thalidomide for 24 hours, the G0/G1 cells increased, the S and G2/M cells decreased, but no significant differences were found in cell-cycle. [Conclusion] Thalidomide can inhibit the growth of CNE-2 cell and increase the radiosensitivity .
出处
《中国肿瘤》
CAS
2009年第3期230-233,共4页
China Cancer
