摘要
参考GenBank已发表的PCV-2基因序列,根据PCV-2(AF027217)和PCV-1(U49186)全基因序列设计合成一对引物,进行PCR扩增,产物经琼脂糖凝胶电泳,呈现一条大小约1 154 bp的特异条带,经条件优化,建立了猪病料中PCV-2感染的PCR检测方法。采用建立的PCR方法对江西各地猪场133份猪的病料进行PCV-2检测,结果表明,受检猪的组织及血清样品中PCV-2的总检出率为53.38%,证实江西各地猪场存在PCV-2感染。
To construct a specific PCR detecting method, a pair of primers were designed to amplify a partial fragment of PCV - 2, referring to PCV - 2 （ AF027217 ） and PCV - 1 （ U49186） complete genome published in Genbank. The PCR product was about 1 154bp in size detected by gelose gel electrophoresis. 133 diseased or dead swine specimens from different pig farms in Jiangxi were detected by the PCR for PCV -2. It showed 71 specimens were positive for PCV -2, the positive rate was 53.38%, it proved that there was PCV -2 infection at different pig farms in Jiangxi.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2009年第1期5-7,共3页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省科技厅2005年重大招标项目(赣科发计字[2005]73号)
江西省教育厅项目(赣教高字[2008]3号)
