摘要
应用微管吸吮技术测量了烧伤血清剌激后２４ｈ内中性粒细胞（ＰＭＮ）与内皮细胞（ＥＣ）粘附力的变化，并应用流式细胞术（ＦＣＭ）检测了烧伤血清作用下介导ＰＭＮ－ＥＣ粘附的主要３个粘附分子：ＣＤ１１ａ／ＣＤ１８、ＣＤ１１ｂ／ＣＤ１８和细胞间粘附因子－１（ＩＣＡＭ－１），以探讨ＰＭＮ－ＥＣ粘附力的分子基础。发现ＰＭＮ在烧伤血清剌激后与正常体外培养的人脐静脉内皮细胞（ＨＵＶＥＣ）间粘附力迅速上升，于１ｈ达到饱和点，并在１２ｈ内保持此水平。烧伤血清剌激ＨＵＶＥＣ后与未受剌激的ＰＭＮ间粘附力逐渐升高，至２４ｈ达峰值，在以后１２ｈ内维持此水平。烧伤血清剌激后，ＰＭＮ其膜表面的ＣＤ１１ａ／ＣＤ１８、ＣＤ１１ｂ／ＣＤ１８迅速表达增强，１ｈ达峰值，在２４ｈ内维持此高表达水平；而ＨＵＶＥＣ受烧伤血清剌激后ＩＣＡＭ－１表达逐渐增多，至１２ｈ达最大值，在以后１２ｈ无明显变化。提示：烧伤血清可剌激ＰＭＮ和ＥＣ分别表达ＣＤ１１ａ／ＣＤ１８、ＣＤ１１ｂ／ＣＤ１８和ＩＣＡＭ－１，从而加强ＰＭＮ－ＥＣ粘附。
This study was to investigate the change of adhesive force between polymorphonuclear neutrophils (PMN) and endothelial cell(EC) in response to burnt patients' serum and its mechanisms in vitro. Adhesive force between a PMN and a HUVEC was calculated by micropipette technique, expressions of CD11a/CD18, CD11b/CD18 and ICAM1 were determined by flowcytometry. The findings were as follows: Adhesive force between a PMN and a HUVEC increased sharply after PMN incubated with burnt serum, which reached the maximum level at 1h after incubation and remained this level in the following 23. However, after HUVEC was stimulated by burnt serum, PMNHUVEC adhesive force increased gradually, reached peak at 12h and remained this level in following 12 h. In response to burnt serum, expressions of CD11a/CD18 and CD11b/CD18 on PMN increased, reached the peak at 1h, and maintained this level within 24 h; expression of ICAM1 on HUVEC increased gradually, reached the peak at 12h. The results suggested that burnt serum could enhance adhesive force between PMN and EC, stimulate expressions of CD11a/CD18,CD11b/CD18 and ICAM1.Therefore,burnt serum played a great role in PMNEC adhesion after burn injury. CD11a/CD18, CD11b/CD18 and ICAM1 were the molecular base of PMNEC adhesion.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1998年第2期98-100,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金
关键词
烧伤
粘附性
中性白细胞
内皮细胞
burns
adhesiveness
neutrophils
endothelial cells
