摘要
研究小麦麦麸碱提物(AHWB)的制备和体外抗氧化活性。小麦麦麸脱淀粉脱蛋白后,碱法水解制备得到3种碱提物(AHWBⅠ、AHWBⅡ和AHWBⅢ),考察其对二苯代苦味酰自由基、羟自由基和超氧自由基的清除作用。3种AHWB均能有效清除DPPH自由基和羟自由基,AHWBⅡ能清除超氧自由基,对于DPPH·,3种碱提物的IC50值分别为0.19、0.54、0.39mg/mL;对于·OH,3种碱提物的IC50值分别为1.13、1.88、1.03mg/mL,其清除效果与Vc的相近;对于O2-·,AHWBⅡ的IC50值为5.98mg/mL。因此小麦麦麸碱提物具有较好的抗氧化作用,可作为天然抗氧化剂。
To study the preparation and antioxidant effect of alkali-hydrolyzed wheat bran （AHWB） in vitro. Three kinds of AHWB （AHWB Ⅰ, AHWB Ⅱ 和 AHWB Ⅲ） were prepared by alkaline hydrolyzing de-starched and de-proteinated wheat bran, and researched on DPPH free radicals, hydroxyl radicals and superoxide radical scavenging. AHWB three can effectively clear the DPPH free radicals and hydroxyl radicals, AHWB Ⅱ be able to clear the superoxide radical. For DPPH, the three alkaline extract of the IC50 values were 0.19 mg/mL 0.54 mg/mL and 0.39 mg / mL respectively, for the · OH, three alkaline extract of the IC50 values were 1.13 mg/mL, 1.88 mg/mL and 1.03 mg/mL respectively, which its effect is similar to that of Vc. For O2^-·, the IC50 of AHWB Ⅱ is 5.98 mg/mL. The results showed that AHWB has good anti-oxidant activity, and can be used as a natural antioxidant.
出处
《食品研究与开发》
CAS
北大核心
2009年第3期56-59,共4页
Food Research and Development
关键词
小麦麦麸碱提物
自由基
抗氧化
alkaline-hydrolyzed wheat bran
free radical
antioxidant effect
