摘要
目的:回顾性分析常规体外受精-胚胎移植(IVF-ET)中受精完全失败的可能原因。方法:选择在2007年1月至2008年8月期间完成的第1次常规体外受精病例,共552对夫妇。男方的精子要求为:妻子取卵当日精液处理后a+b级精子总数>1×106,在术前1年内,按照Kruger/Tygerberg标准,精子畸形率<96%者。结果:552对夫妇中,515对常规体外受精成功,占93.3%,其受精率为:78.67%,此组为受精组;37对受精完全失败,发生率为6.7%,为受精失败组。将两组进行比较,受精失败组中原发不孕和畸形精子症的比例显著高于受精组(P<0.05),而两组促排卵天数、获卵数、促性腺激素用量、男女方年龄、长短方案的应用及少弱精子症的比例均没有显著性差异。结论:按照Kruger严格评分法对于男方畸形精子症者,应考虑行部分卵子ICSI,以避免受精失败。
Objective： To analyze the possible causes of total fertilization failure （TFF） in conventional in vitro fertilization （IVF）. Methods ： We included in this study 552 couples who accomplished the first conventional IVF cycle in our center from January 2007 to August 2008. All the males met the Kruger/Tygerberg criteria, with the teratozoospermia rate 〈 96% in the previous 12 months and the postwash motile sperm 〉 1 million on the day of egg retrieval. The eggs were fertilized totally by conventional IVF. Results ： Of the total number, 515 couples got zygotes by conventional IVF, with a fertilization rate of 78.67% , and 37 suffered TFF. The rates of primary infertility and teratozoospermia were significantly higher in the TFF group than in the fertilized group （ P 〈 0.05 ）. There were no statistically significant differences between the two groups in the number of controlled ovarian hyperstimulation （COH） days, the number of oocytes retrieved, the dose of gonadotropin （Gn） used, the age of the couples, the length of protocols and the rate of oligoasthenozoospermia. Conclusion： Intracytoplasmic sperm injection （ICSI） can be considered for at least some of the oocytes to avoid unnecessary fertilization failure in teratozoospermia patients by Kruger / Tygerberg strict criteria.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2009年第2期106-109,共4页
National Journal of Andrology
基金
陕西省科技厅科技攻关资助课题[2007K14-02(1)]~~
