摘要
从疑似猪生殖与呼吸综合征病毒(PRRSV)感染猪的肺、淋巴结提取样本RNA,针对PRRSV的部分Nsp2基因进行了RT-PCR扩增。结果显示,扩增得到大小不同的2种(HW1和HW2)DNA条带。对PCR产物进行序列分析的结果表明,HW1和HW2都是Nsp2基因的扩增产物,HW2大小为1 062 bp,相对于经典PRRSV缺失90 bp,HW1为975 bp,缺失177 bp,二者之间的核苷酸同源性为98.2%;氨基酸同源性为96.6%。这说明病猪混合感染了2种Nsp2基因不同程度缺失的PRRSV。
Two different fragments（HW1 and HW2） were amplified by RT-PCR from lung and lymph node of pigs infected suspectably with PRRSV. Sequence analysis indicated that both HWl and HW2 were originated from PRRSV strains, HW2 was 1 062 bp and deleted 90 bp compared with the Nsp2 gene of classical PRRSV strains,and HWl was 975 bp and deleted 177 bp. The HWl shared 98.2% homology with HW2 at nucleotide level and 96.6G at amino acid level respectively. The results showed that co-infection of PRRSV strains with different gene deletion in Nsp2 gene site occurred in the infected pigs.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2009年第2期150-153,共4页
Chinese Veterinary Science
基金
贵州省"十一五"农业科技攻关重大项目子课题[黔科合NZ字(2005)3002]
贵阳市农业局科技计划项目(2007-08)
贵阳市农业局科学技术计划项目([2008]动防-03号)
