摘要
目的应用蛋白质组学方法在患者血清中寻找与克罗恩病相关的蛋白质。方法采取克罗恩病患者以及正常成人血清蛋白样本各4例,用不同的CyDye荧光染料标记后进行胶内差异双向凝胶电泳(2-DDIGE),并对获得的图谱进行分析及对差异蛋白质进行基质辅助激光解吸离子化飞行时间质谱鉴定。结果通过2-DDIGE分析,发现克罗恩病患者中存在29个表达异常蛋白质点,其中胶号为973的蛋白质点,在克罗恩病患者患者血清中比正常成人组表达升高2.55倍(P<0.05),该蛋白点经质谱鉴定为CD45。结论CD45在克罗恩病患者血清的高表达提示在克罗恩病的病程中免疫系统失衡可能起着一定作用。
Objective To search for differentially expressed proteins in the serum of patients with Crohn＇s disease. Methods Serum protein samples obtained from 4 patients with Crohn＇s disease and 4 normal adults were cross-labeled with different CyDyes and underwent two-dimensional differential in-gel electrophoresis （2-D DIGE） and imaging analysis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） was used to identify the differentially expressed proteins. Results 2-D DIGE revealed that the protein on spot 973 was overexpressed by 2.55 folds in the serum of patients with Crohn＇s disease compared with that in normal adults （P〈0.05）. The protein was identified as CD45 using mass spectrometry. Conclusion CD45 overexpression in the serum of patients with Crohn＇s disease may play a role in the disequilibrium of the immune system.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第2期259-263,共5页
Journal of Southern Medical University
基金
广东省自然科学基金(06104601)
广东省自然科学基金博士启动项目(06300805)
关键词
克罗恩病
双向胶内差异凝胶电泳
基质辅助激光解吸离子
质谱
CD45
Crohn＇s disease
two-dimensional differential in-gel electrophoresis, two-dimensional
matrix-assisted laser desorption/ionization mass spectrometry
CD45
