摘要
目的探讨四氮唑蓝比色分析法(MTT法)用于指导临床个体化化疗药物选择的实用性,为乳腺癌临床化疗用药提供参考。方法采用MTT法测定乳腺癌细胞株MCF-7和MDA-MB-435S对不同浓度的化疗药物5-氟脲嘧啶(5-Fu)的体外敏感性。结果不同浓度的5-Fu对高转移和低转移乳腺癌细胞株的生长均有一定的抑制作用,且抑制率呈剂量依赖性。结论MTT法检测有助于指导临床选择化疗药物剂量,提高化疗效果,减少毒副作用,为乳腺癌临床个体化化疗方案的选择提供客观依据。
Objective To assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of breast cancer cells to 5 fluorouracil （5-Fu）. Methods The chemosensitivity of human breast carcinoma cell lines MCF-7 and MDA-MB-435S to 5-Fu at different concentrations was evaluated with MTT assay. Results 5-FU treatment resulted in dose-dependent growth inhibition of the breast cancer cells with both low and high metastatic capacities. Conclusions MTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for breast cancer.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第1期97-99,共3页
Journal of Southern Medical University
