摘要
目的揭示克山病心肌间质中胶原的类型、分布、排列与含量。方法采用本室克山病尸检材料，应用苦味酸－天狼星红染色和偏振光显微镜观察和图像分析技术，系统地研究了克山病心肌胶原的变化。结果在急性克山病的瘢痕灶内，胶原排列成疏网状，Ⅲ型胶原比例增加。慢性克山病的瘢痕中，胶原排列致密，Ⅰ型胶原比例增加，Ⅰ／Ⅲ型胶原比值增大。在心肌细胞非坏死区域胶原含量明显高于对照组，出现间质纤维化，以慢性克山病最明显。亚急性和慢性克山病的心内膜厚度和血管周围胶原体密度明显高于对照组。结论在克山病心肌细胞变性坏死的同时伴随着胶原代谢的紊乱，胶原蓄积，心肌间质胶原发生重构，是克山病心功能不全的重要因素之一。
Objective To clarify the localization, distribution, content and type of collagen in the myocardium of patients with Keshan disease (KD). Methods Picrosirium red staining, polarization microscopy and image analysis were used to study autopsy specimens from 335 KD patients. Results A loose network of collagen and a higher ratio of type Ⅲ collagen were found in the myocardial scar tissues of acute KD patients. In the myocardial scar tissues from chronic KD patients, the collagen fibers were dense, the ratio of type Ⅰ collagen was higher, the ratio of type Ⅰ to type Ⅲ is increased in chronic KD and decreased in acute KD. The amount of collagen in non necrotic regions was significantly higher than that of the controls. Conclusion The degeneration and necrosis of myocytes in KD is associated with disorders of collagen metabolism and with structural modelling of collagen.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1998年第1期13-16,共4页
Chinese Journal of Pathology
