摘要
采用磷酸钙－ＤＮＡ共沉淀法，将ｐＲＥＰ－８－ＩＦＮ－表达质粒直接注射至小鼠腹腔，可成功地转染腹腔巨噬细胞（ＭΦ），并表达其基因产物，能有效地抑制肿瘤生长；再次基因转染，可获得增强的抗肿瘤效应。还发现转基因ＭΦ所分泌的激活因子能激活未转基因的ＭΦ；增加腹腔ＭΦ的数量；增强ＭΦ的杀瘤活性；并使ＭΦ分泌一定水平的ＮＯ、ＩＬ－１和ＴＮＦ等，进一步增强抗肿瘤作用。转基因ＭΦ及其表达产物可使脾细胞ＮＫ活性和内源性ＬＡＫ活性增强，提高机体抗肿瘤功能，为肿瘤的细胞因子基因治疗提供了一种简便而有效的抗肿瘤新途径。
he expression vector pREP8IFN was injected i.p.into mice with calcium phosphate coprecipitation method and IFN gene was successfully transfected into peritoneal MΦ whose experssion products could inhibit the tumor growth effectively. After gene retransfection, the enhanced antitumor effects would be gained. It was found that active factors secreted by transgenic MΦ can activate untransgenic MΦ, increase the numbers of MΦ, secrete some NO, IL1 and TNF, and exert high tumoricidal activity. The transgenic MΦ and its expressive products can increase the NK and endogenous LAK activity significantly and activate the antitumor immunity in vivo. This study would provide a new effective approach to treat tumor by cytokine gene therapy.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第1期22-26,共5页
Chinese Journal of Immunology
关键词
IFN-Γ基因
基因转染
巨噬细胞
抗肿瘤
IFN gene Gene transfection Macrophage(MΦ) Antitumor effect Mouse
