摘要
Ｕ９３７细胞、经ＰＭＡ及ｄｂｃＡＭＰ预处理的Ｕ９３７细胞，用ｒｈＣ５ａ、ＰＭＡ、Ａ２３１８７分别诱导，以ＥＬＩＳＡ法测定细胞培养上清中ＩＬ－８，证实：ｒｈＣ５ａ不能诱导Ｕ９３７细胞产生ＩＬ－８，但可诱导经ＰＭＡ、ｄｂｃＡＭＰ预处理的Ｕ９３７细胞产生ＩＬ－８；用ＰＭＡ激活ＰＫＣ和（或）以Ａ２３１８７提高细胞内Ｃａ２＋浓度可使ＩＬ－８分泌水平增高。研究结果有助于加深对补体系统与细胞因子之间关系的认识，以及探索减轻Ｃ５ａ致病作用新途径。
he interleukin8 in the supernatants of U937 cells,PMA or dbc AMP pretreated U937 cells which were induced with rhC5a,PMA and A23187 were detected by ELISA.Stimulated with rhC5a,U937 didn′t secrete detectable IL8,but PMA or dbc AMP pretreated U937 cells secreted detectable IL8.It was discovered that activation of PKC with PMA and(or) rise in cytosolic Ca2+ with A23187 increased secretion of IL8.The result contributed to finding a new way to reduce the pathogenesis of C5a and getting a deeper understanding of the relationship between complement system and cytokines.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第1期12-14,共3页
Chinese Journal of Immunology
关键词
C5A
U937细胞
白细胞介素8
钙离子
载体
Complement 5a(C5a) U937 cells Interleukin8(IL8) Phorbol ester Calcium ionophore
