摘要
目的通过免疫组织化学方法分析平痫冲剂对戊四唑(PTZ)致痫大鼠海马氨基酸类神经递质受体的影响,探讨其抗癫痫的作用机理。方法将60只28日龄的Wistar大鼠随机分为正常对照组(A组)、模型组(B组)、苯巴比妥组(C组)及平痫冲剂小(D组)、中(E组)、大(F组)剂量组。除正常对照组外,其余各组大鼠腹腔注射PTZ复制慢性癫痫模型,通过免疫组织化学方法和显微图像分析技术分别检测各组大鼠海马CA1、CA3的谷氨酸受体(NMDAR1)和γ-氨基丁酸受体(GABA-ARα1)免疫反应的阳性细胞数(n)、阳性细胞面积比(Aa%)及阳性细胞积分吸光度(IA)。结果与A组比较,B组海马CA1、CA3等部位NMDAR1的n、Aa%上升,IA增加(P<0.01);与B组比较,C组及D、E、F组不同部位NMDAR1的n、Aa%都有不同程度下降,IA也有不同程度减少(P<0.05)。同A组比较,B组海马CA1、CA3等部位GABA-ARα1的n、Aa%下降,IA降低(P<0.01);与B组比较,C、D、E、F组不同部位GABA-ARα1的n、Aa%都有不同程度增加,IA也有不同程度升高(P<0.05)。结论平痫冲剂抗癫痫的作用机理可能是通过抑制海马兴奋性神经递质受体NMDAR1的活性与表达,并激活其抑制神经递质受体GABA-ARα1的活性与表达,从而降低大脑皮质的兴奋性,有效抑制癫痫的形成及发展。
Objective Though immunohistochemical study on the effects of Pingxian Powder on N-methyl-D-aspartate receptorl （NMDAR1） and γ-aminobutyric acid-A receptor α1 （GABA-AR α1） in central nervous system of pentylenetetrazol-induced epileptic rats, to explore its possible antiepileptic mechanism. Methods Sixty wistar rats were divided into control group （A）, model group （B）, phenobarbital group （C） and Pingxian Powder small （D）, middle （E）, Large （F） dosage group. Pentylenetetrazole （PTZ） was injected into abdomen of rat to reconstruct epileptic kindling model. The total number, percentage of positive area and integral optical density of NMDAR1 and GABA-AR α1 of immunoreactive cells in hippocampus CA1, CA3 were studied by the immunohistochemical method and image analyzing system. Results The total number, percentage of positive area and integral optical density of NMDAR1 of immunoreactive cells in hippocampus formation of Group B were more than Group A （P〈0.01）, while these numerical values of Group C, D, E, F decreased to varying degrees compared with Group B （P 〈0.05）. The total number, percentage of positive area and integral optical density of GABA-AR α1 of immunoreactive cells in hippocampus formation of Group B were less than Group A （P〈0.01）, while these numerical values of Group C, D, E, F enhanced to varying degrees compared with Group B （P 〈0.01, P 〈0.05）. Conclusions Pingxian Powder may reduce the excitability of cerebral cortices by inhibiting the activities of NMDAR1 and promoting the activities of GABA-AR α1 in hippocampus to inhibit the formation and development of epilepsy.
出处
《中国中医药信息杂志》
CAS
CSCD
2009年第3期27-29,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
甘肃省教育厅科研项目(0505-04)
