摘要
目的:观察孕酮对于吗啡所致奖赏效应及相关脑区中亮氨酸脑啡肽水平的影响。方法:将40只SD大鼠随机均分为对照组(环糊精)、吗啡组、孕酮组和孕酮+吗啡组,建立吗啡条件性位置偏爱(CPP)模型,上午各组皮下给予相应药物后放入白室训练45min;下午各组均皮下和腹腔给予等量的环糊精和生理盐水后放入黑室训练。连续训练10d后进行CPP测试并处死大鼠取脑组织采用放射免疫法测定大鼠不同脑区中亮氨酸脑啡肽(L-EK)的含量。结果:与对照组比较,吗啡组发生CPP效应,下丘脑、伏隔核和额叶皮质中的L-EK水平显著降低(P<0.05或P<0.01);与吗啡组比较,孕酮+吗啡组CPP效应受到抑制,上述位置中L-EK水平显著升高(P<0.05或P<0.01),而海马和中脑内L-EK水平未见显著性变化。结论:孕酮可以有效抑制吗啡CPP效应,其机制可能与其逆转吗啡诱导的相关脑区中的L-EK水平的变化有关。
OBJECTIVE： To investigate the effects of progesterone on morphine rewarding effect and levels of leuenkephalin （L EK） in rat brain. METHODS： 40 male SD rats were randomly assigned to control group （cyclodextrin）, morphine group, progesterone group and progesterone plus morphine group to establish morphineinduced conditioned place preference （CPP） rat model. In the morning, all rats received training for 45 min in white room after being hypodermically injected with the corresponding drugs. And in the afternoon, all rats received the same training in black room after being hypodermically injected with cyclodextrin and intraperitoneal injected with saline of the same dose. 10 days later, CPP test was conducted to investigate the morphine rewarding effect, and radioimmunoassay was established to determine the levels of L- EK in rat brain. RESULTS： Compared with control group, morphine successfully induced the CPP effect and the levels of L- EK following morphine administration were decreased in hypothalamus, nucleus accumbens septi and cortex of frontal lobe （P 〈 0.05 or P 〈 0.01）. In morphine plus progesterone group as compared with morphine group, the CPP effect was inhibited and levels of L - EK in hypothalamus, nucleus accumbens septi and cortex of frontal lobe were significantly increased （ P 〈 0.05 or P 〈0.01）, but no difference of which in hippocampus and midbrain were not significant. CONCLUSION： The inhibitory effect of progesterone on morphine - induced CPP might be related to its regulation on L - EK.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第7期495-497,共3页
China Pharmacy
基金
河北省自然科学基金资助项目(C200500834)
关键词
孕酮
吗啡
大鼠
条件性位置偏爱
亮氨酸脑啡肽
Progesterone
Morphine
Rat
Conditioned place preference
Leu - enkephalin
