摘要
目的探索抗ＤＮＡ酶Ｂ测定Ａ组溶血性链球菌（ＧＡＳ）感染血清学方法的诊断价值。方法用抗ＤＮＡ酶Ｂ微量法和自制试剂，测定５４８例ＧＡＳ感染所致疾病的小儿血清抗ＤＮＡ酶Ｂ抗体，同时测定抗链球菌溶血素Ｏ（ＡＳＯ）。结果（１）急性风湿热组（ＡＲＦ）抗ＤＮＡ酶Ｂ阳性率（７２．５％）与ＡＳＯ阳性率（７６．５％）比较，差异无显著意义（Ｐ＞０．０５）；但活动期风湿性心脏病（简称风心病）组病人的抗ＤＮＡ酶Ｂ阳性率（８２．６％）明显超过ＡＳＯ阳性率（５５．４％）（Ｐ＜０．００１）；抗ＤＮＡ酶Ｂ联合ＡＳＯ测定在两组的阳性率均在９０％左右。（２）急性肾小球肾炎（ＡＧＮ）皮损组抗ＤＮＡ酶Ｂ阳性率９２．６％，明显超过ＡＳＯ的５９．２％（Ｐ＜０．０５）。（３）ＡＧＮ上感组抗ＤＮＡ酶Ｂ与ＡＳＯ的阳性率分别为７６．７％和７３．９％（Ｐ＞０．０５）。抗ＤＮＡ酶Ｂ联合ＡＳＯ的阳性率在上感组和皮损组均超过９０％。结论抗ＤＮＡ酶Ｂ在诊断ＡＲＦ和ＡＧＮ方面具有重要的临床价值，特别与ＡＳＯ联合应用价值更大；对活动期风心病和ＧＡＳ皮肤感染致ＡＧＮ的诊断价值明显优于ＡＳＯ。
Objective To evaluate diagnostic value of anti DNase B microtitration in group A streptococcal (GAS) infection. Methods Anti DNase B antibodies of 548 children with GAS infection were detected with anti DNase B test. Antistreptolysin “O” (ASO) was simultaneously measured. Results (1) No significant difference was observed between positive rate of anti DNase B (72.5%) and ASO (76.5%) in acute rheumatic fever (ARF) group ( P >0.05). However, the positive rate of anti DNase B (82.6%) was significantly higher than that of ASO (55.4%) in acute rheumatic heart disease (ARHD) group ( P <0.001). When the anti DNase B test was studied simultaneously with the ASO test, the number of sera with elevated titers in at least one test increased to about 90% in ARF and ARHD groups; (2) The positive rate of anti DNase B test (92.6%) was obviously higher than that of ASO (59.2%) in pyoderma subgroup of acute glomerulonephritis (ANG) cases ( P <0.05). However, there was no significant difference between anti DNase B positive rate (76.7%) and ASO (73.9%) in upper respiratory tract infection subgroup of such cases ( P >0.05). The positive rate exceeded 90% when anti DNase B was used together with ASO in AGN group. Conclusion Anti DNase B microtitration test may be an useful tool in the diagnosis of ARF and AGV. The positive rate of anti DNase B test was higher than that of ASO in patients with ARHD and those with AGN with underlying skin infection.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1998年第3期168-170,共3页
Chinese Journal of Pediatrics
关键词
儿童
诊断
溶血性链球菌
风湿热
抗DNA酶B
Streptococcus, pyogenes Streptococcal infections Rheumatic fever Glomerulonephritis Deoxyribonucleases
