摘要
作为一种将被用作创面修复的医用生物材料,丝素蛋白是否影响血管内皮生长因子(Vascular endothelial growth factor,VEGF)的转录表达是一个值得关注的问题。本研究通过对ECV304细胞在再生丝素膜上形态学观察和生长曲线的测定,以及采用酶联免疫吸附实验(Enzyme linked i mmunosorbent assay,ELISA)证实再生丝素膜不影响ECV304细胞的生长和功能的正常发挥。转入人VEGF基因的L929细胞在再生丝素膜上生长,ELISA检测和实时定量RT-PCR(Real-time quantitative reverse transcriptase polymerase chain reaction,Real-time quantitative)证实了再生丝素膜不影响该转基因细胞VEGF基因的转录与表达。再生丝素膜对组织修复过程中诱导血管生成无任何不良影响,是一种安全有效的医用生物材料。
As a biomaterial to be used for reparation in the case of trauma, the silk fibroin, particularly its effect on the transcription and expression of VEGF gene, is a concern. In this study, the ECV304 cell＇s growth shape and growth curve on the regenerated silk fibroin film were observed, and its VEGF secretion level was measured by ELISA test. It was found that the regenerated silk fibroin film did not interfere with ECV304 cell＇s growth and func- tion. The L929 cell transfected with human VEGF gene grew on the regenerated silk fibroin film; the real-time quan- titative RT-PCR method and ELISA test were used for detecting the transcription and expression of VEGF gene. The results showed the regenerated silk fibroin film did not interfere with the transcription and expression of VEGF gene. Therefore, the regenerated silk fibroin film is a safe biomaterial for inducing vascularization with no untoward effect on the reparation of trauma.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2009年第1期110-115,共6页
Journal of Biomedical Engineering
基金
国家重点基础研究发展计划项目(973计划)资助项目(2005CB623906)
关键词
丝素蛋白
再生丝素膜
血管内皮生长因子
实时定量RT—PCR
Silk fibroin Regenerated silk fibroin film Vascular endothelial growth factor（VEGF） Real-time quantitative RT-PCR
