摘要
目的探讨痰中细胞因子、嗜酸细胞阳离子蛋白（ＥＣＰ）水平与哮喘严重程度的关系。方法采用酶联免疫法及荧光免疫法对轻（Ｍ组１０例）、中、重度（ＭＳ组１５例）哮喘患者痰液白细胞介素（ＩＬ）５、肿瘤坏死因子α（ＴＮＦ－α）、可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）、ＥＣＰ水平进行检测。结果中、重度哮喘患者痰液ＩＬ－５（３５ｎｇ／Ｌ以上）、ＴＮＦ－α（Ｍ组为１４９±５９ｎｇ／Ｌ，ＭＳ组为２６７±１４７ｎｇ／Ｌ）、ｓＩＬ－２Ｒ（Ｍ组为３４８±１０７ｋＵ／Ｌ，ＭＳ组为４８８±１２７ｋＵ／Ｌ）、ＥＣＰ（Ｍ组为１２７±９５μｇ／Ｌ，ＭＳ组为２７８±１５０μｇ／Ｌ）浓度均显著高于轻度哮喘患者。结论哮喘患者痰液中可检出有关的细胞因子及炎性介质，它们参与了哮喘的急性发作并反映哮喘的严重程度。
Objective To examine whether levels of inflammatory cytokines and eosinophil cationic protein (ECP) in the sputum reflect the severity of bronchial asthma. Method We collected sputum expectorated spontaneously from 15 asthmatics with acute attacks of moderate to severe degree (MS group) and 10 subjects with acute attacks of mild asthma (M group). The interleukin (IL) 5(35 ng/L) tumor necrosis factor (TNF) α(M 149±59 ng/L, MS 267±147 ng/L), soluble IL 2 receptor (sIL 2R) (M 348±107 kU/L, MS 488±127 kU/L) levels in the sputum were measured with enzyme linked immunosorbent assay, and sputum ECP (M 127±95 μg/L, MS 278±150 μg/L) concentration were measured by Immuno CAP System. Result Sputum IL 5、TNF α、sIL 2R、ECP concentrations in moderate to severe patients were significantly higher than in mild subjects.Conclusion These findings suggest that inflammatory cytokines and mediator levels are detectable in the sputum from asthmatics and they might particrpate in the exacerbation of asthma.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1998年第2期108-110,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
全军医药卫生科研基金
