摘要
目的探讨神经生长因子(NGF)和碱性成纤维细胞生长因子(bFGF)分别及联合作用对体外培养的人牙周膜细胞(hPDLCs)增殖的影响。方法体外培养人牙周膜细胞,与不同浓度的NGF、bFGF或NGF+bFGF作用,用噻唑盐(MTT)法观察PDLC增殖的情况。结果不同浓度的NGF、bFGF都可促进人PDLC的增殖,这种促进作用呈一定的浓度依赖性。NGF与bFGF联合应用对人PDLC的增殖有协同作用,且与单独应用相比具有统计学差异。结论NGF、bFGF都可促进人PDLC的增殖,且二者联合具有协同作用。
Objective To evaluate the effects of nerve growth factor（NGF） and basic fibroblast growth factor（bFGF） on proliferation of human periodontal ligament cells（hPDLCs） cultured in vitro. Methods Human periodontal ligament cells were cultured by explant separation method. The fifth to seventh generation hPDLCs were＇cuhured with the different concentrations of NGF, bFGF and NGF ＋ bF- GF. The proliferation of hPDLCs was measured by MTT colorimetric assay. Data was analyzed by ANOVA. Results Proliferation of hPDLCs increased in a dose-dependent manner after cultured with NGF or bFGF. The NGF and bFGF had synergetic effect on proliferation of hPDLCs. Conclusion NGF and bFGF can be used as bioactive mediators for periodontal regeneration.
出处
《山西医科大学学报》
CAS
2009年第1期50-52,共3页
Journal of Shanxi Medical University
