摘要
以2-酮基-D-葡萄糖酸产生菌Ac9为出发菌株,采用紫外线诱变与添加噬菌体进行筛选相结合的方法,获得了两株抗性菌株Au4-2和Af3。在种子培养基中含有噬菌体的条件下,31℃,摇瓶培养24h,抗性菌株Au4-2与Af3的菌体生长光密度值OD650×20分别为0.74,0.55。镜检显示菌体量多,呈短杆状;而对照菌株Ac9的菌体光密度值则为0.14,镜检显示菌体量少,呈球状。在发酵培养基中添加噬菌体的条件下,31℃,摇瓶培养72h,抗性菌株Au4-2与Af3产酸分别可达15.45g/100mL,14.55g/100mL;而对照菌株则几乎没有产酸。
The two phage-resistant strains Aug-2 and At3 were obtained from the mutation of the 2-keto-D-gluconic acid producing strain Ac9 which had been treated by U.V. light as a mutagen with adding phages in the culture media to weed out the no phage-resistant strains. In the condition of the phages in the seed culture media, shaking flask at 31℃ for 24 h, Au4-2 and At3＇ s OD650 ×20 were 0.74 and 0.63 separately while it showed that there were many bacteria in the form of short-pole under the microscope, But AC9＇s 0D650×20 was 0.14 and only a few sphericity bacteria. In the condition of the phages in the fermentation culture media, shaking flask at 31 ℃ for 72 h,the yields of 2-keto-D-gluconic acid by Au4-2 and Af3 are 15.45 g/100mL and 14.55 g/100mL, separately, and that the control strain could hardly produce any acid.
出处
《科学技术与工程》
2009年第5期1236-1239,共4页
Science Technology and Engineering
