摘要
高脂饲料喂饲大鼠建立高脂血症模型,同时给予西红花苷治疗,实验结果揭示显著降低TG、TC、LDL-C、VLDL-C。脂质负荷实验表明,西红花苷降血脂同时抑制脂肪和胆固醇吸收,粪中脂质检测发现西红花苷加速脂肪和胆固醇的粪排泄,但对胆汁酸没有影响。胆固醇肠管吸收实验表明西红花苷不能直接抑制胆固醇在肠道的转运,西红花苷对脂肪吸收的关键酶胰脂肪酶有较强的抑制作用。因此西红花苷可能通过抑制胰脂肪酶阻碍脂肪吸收,由于胆固醇吸收依赖脂肪的吸收,所以胆固醇吸收同样受到抑制。
The hypolipidemic mechanism of crocin, an active ingredient in Gardenia jasminoides Ellis and Crocus sativus L, was examined in rats. In diet-induced hyperlipidemic rats, a 10-day treatment with crocin significantly reduced serum triglyceride, total cholesterol, low density lipoprotein （LDL） cholesterol and very low density lipoprotein （VLDL） cholesterol level in the daily dose range of 25 to 100 mg/kg. Results of the modified fat-loading method indicated that crocin inhibited the absorption of fat and cholesterol and this inhibition is closely related to the hydrolysis of fat. In addition, the modified fat-balance method demonstrated that crocin increased the fecal excretion of fat and cholesterol in rats, but had no influence on the elimination of bile acids. The results of the in situ loop method and enzyme assay indicated that crocin could not directly block the absorption of cholesterol from rat jejunum but could selectively inhibit the activity of pancreatic lipase as a competitive inhibitor. These findings suggest that crocin yielded its hypolipidemic effect by inhibiting pancreatic lipase, leading to the malabsorption of fat and cholesterol.
出处
《中国执业药师》
CAS
2009年第2期10-16,22,共8页
China Licensed Pharmacist
