摘要
目的了解肺癌组织中巨噬细胞浸润情况,探讨肺癌组织中肿瘤相关巨噬细胞(tumor associated macrophages,TAMs)分布及其与肺癌患者预后的关系。方法采用免疫组织化学链霉素生物素法(LSAB)检测136例肺癌癌巢和癌间质中CD68巨噬细胞数,结合随访资料分析TAMs与患者术后生存的关系。结果肺癌组织及癌间质中大量TAMs浸润,肺癌间质中TAMs表达显著高于癌巢(36.00个/HFP vs.23.80个/HFP,P<0.05)。癌巢内TAMs均数在Ⅰ+Ⅱ期显著高于Ⅲ+Ⅳ期(32.60个/HFP vs.14.80个/HFP,P<0.05),而癌间质TAMs均数在Ⅰ+Ⅱ期显著低于Ⅲ+Ⅳ期(24.30个/HFP vs.47.60个/HFP,P<0.05);肺癌癌巢TAMs和癌巢/癌间质TAMs比值与生存时间呈正相关?rs=0.510,0.633),而癌间质TAMs与生存时间呈负相关(rs=-0.187);5年生存率在癌巢TAMs高密度者显著高于低密度者(51.4%vs.11.1%,P<0.05),而癌间质TAMs高密度者显著低于低密度者(18.9%vs.44.4%,P<0.05),癌巢TAMs/癌间质TAMs比值大于中位数者显著高于小于中位数者(58.1%vs.4.2%,P<0.01)。结论Cox风险比例模型分析显示:癌巢TAMs和癌巢/癌间质TAMs比值越大则肺癌患者生存率越高,而癌间质TAMs越大则肺癌患者生存率越低。癌巢TAMs、癌间质TAMs及癌巢/癌间质TAMs比值均可作为肺癌患者的预后指标。
Objectives To investigate the effects of the distribution of tumor associated macrophages （TAMs） on prognosis in the patients with non-small cell lung cancer. Methods The number of CD68＋ macrophages in 136 lung cancer nest and stroma was counted simultaneously by labelled streptavidin biotin method（LSAB）, and its correlation with patient postoperation prognosis was analyzed. Results CD68 macrophas were observed in both inside and around the cancer tissue,The mean TAMs in cancer stroma （36.00/HFP） was higher than that in cancer nest （23.80/ HFP,P〈0.05）. Mean TAMs in nest of stage Ⅰ ＋ Ⅱ cancer was significantly higher than that of stage Ⅲ ＋Ⅳ cancer （32.60/HFP vs. 14.80/HFP, P〈0. 05） ,and mean TAMs in stroma of stage Ⅰ ＋ Ⅱ cancer was significantly lower than that of stage Ⅲ ＋Ⅳ cancer（24.30/HFP vs. 47.60/HFP,P〈0.05）. The number of TAMs in cancer nest and the ratio of nest TAMs/stoma TAMs were both positively correlated with the patient survival time （rs =0. 510, 0. 633, respectively）. Otherwise the number of TAMs in cancer stroma was negatively correlated with the patient survival time （rs = -0. 187）. Five-year survival rate in patients with high density TAMs in cancer nest was significantly higher than that in patients with low density TAMs （51.4% vs. 11.1%, P〈0.05）, while reverse correlation between TAMs in cancer stroma and patient 5-year survival rate was observed （18.9% vs. 44.4%, P〈0.05）. And 5-year suvival rate in patients with high ratio of nest/stroma TAMs was higher than that with low ratio （58. 1% vs. 4.2%, P〈0.01）. Conclusion Cox regressive prognostic analysis showed that the higher the nest/stroma TAMs ratio, the higher probability of the patients survival time. While the higher number of TAMs in the cancer stroma, the lower probability of the patients survival time. Our results showed that distribution pattern of TAMs in cancer nest and cancer stroma could possibly be used to estimate the prognosis of patients with non-sma
出处
《中国胸心血管外科临床杂志》
CAS
2009年第1期43-47,共5页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
关键词
肿瘤相关巨噬细胞
预后
非小细胞肺癌
Tumor associated macrophage
Prognosis
Non-small cell lung cancer
