摘要
目的研究哮喘患者血清被动致敏后,细胞外信号调节激酶(extracellular signal regulate dkinase,ERK)磷酸化的变化,以探讨ERK信号传导途径在哮喘发病中的作用。方法免疫细胞化学法及免疫印记法检测ERK蛋白及其磷酸化(p-ERK)水平。结果哮喘患者血清被动致敏后,ERK蛋白的表达无明显变化,而p-ERK的表达明显升高(n=4,P<0.05)。结论哮喘患者血清被动致敏后,HASMCs的ERK磷酸化显著增强,此变化可能是哮喘形成和发生的机制之一。
[Objective] To investigate the effect of passive sensitization by asthmatic serum on the extracellular signal regulated kinase （ERK） in human airway smooth muscle cells （HASMCs）. [Methods] Immunoeytochemistry staining and Western-blot techniques were used to detect the protein ERK and level of Phospho-ERK （p-ERK）. [Results] There was no significance in protein ERK between control and passively sensitized groups. But the level of p-ERK in passively sensitized group was significantly higher than：that in control groups （n =4, P 〈0.05）. [Conclusion] P-ERK was significantly increased in HASMCs passively sensitized by asthmatic serum compared with nonsensitized cells. This change might be involved in the mechanisms of the formation and development of asthma.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第3期424-426,共3页
China Journal of Modern Medicine
基金
河南省科技攻关项目(No:072300450100)
河南省卫生厅医学高新技术发展扶持项目(No:20060140)
关键词
哮喘
细胞外信号调节激酶
信号传导
asthma
extraceUular signal regulated kinase （ERK）
signal transduction
