摘要
堆肥过程中接种分解或转化能力较强的微生物,可以加速堆肥的腐熟和改善产品品质。利用无机磷选择培养基,从添加磷矿粉的高温阶段堆肥样品中,分离筛选出6株菌落和透明圈较大的高温无机磷降解菌,经耐热性实验,结果其中的2株(No.C、No.D)温度适应范围较广,在25～55℃之间均可生长。对这2株菌进行无机磷培养基摇瓶培养实验,测定了其高温下的解磷能力。结果表明,所筛选出的无机磷降解菌,在50℃高温培养下,发酵液中的可溶性磷含量分别于第14、16d达到最大值,为263.8和242.0μg.mL-1,对磷矿粉的最大降解率分别为32.35%和29.78%。通过形态学观察、生理生化指标鉴定,初步确定分离到的这2株具有较高活性的高温无机磷降解菌分别为软化芽孢杆菌属(Bacillus.Macerans)和巨大芽饱杆菌属(Bacillus.Megaterium)。
To inoculate microorganisms which can decompose or transform in composting process can accelerate the decay of compost and im- prove the quality of composting product. Six microorganisms those could decompose inorganic phosphorus were separated from high tempera- ture stage composting samples, which had bigger colonies and transparent circle in selective culture media of inorganic phosphorite powder. And thermally stable experiments indicated that 2 of those 6 microorganisms, which numbered C and D, had broad temperature adaptation scope and could live in the temperature from 25-55 ℃. Cultivation experiments under inorganic phosphorus medium were carried out to test the capacities of decomposing phosphorus of the two organisms under high temperature, which indicated that under high temperature of 50 ℃, dissolved phos- phorus in fermentation fluid reached maximum value of 263.8 μg·mL-1 and 242.0 μg·mL-1 after 14 days and 16 days respectively. The maximum decomposing rates of phosphorite powder of the two microorganisms were 32.35% and 29.78%. Through morphological observation and appraisal of physiological and biochemical index, these two microorganisms were Bacillus. Macerans and Bacillus.Megaterium respectively.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2009年第2期393-397,共5页
Journal of Agro-Environment Science
基金
国家重点基础研究发展规划(973)项目(2005CB724203)
哈尔滨学科后备带头人基金项目(2005AFXXJ043)
国家科技支撑计划课题项目(2006BAC06B04)
农业科技成果转化资金项目(2006GB24420465,2007GB24420459,2008GB24420470)
国家自然科学基金(50878201)
关键词
高温堆肥
无机磷
降解
分离
鉴定
high-temperature compost
inorganic phosphorus
degeneration
separation
appraisal
