摘要
目的探讨多器官功能障碍综合征(MODS)病程中脾脏树突状细胞(DC)变化对炎症因子的影响与作用。方法采用腹腔注射酵母多糖的方法复制小鼠MODS模型,分为正常对照组和MODS组。采用流式细胞技术检测脾脏DC免疫表型,运用免疫组化技术检测脾脏中促炎因子IL-1β与抗炎因子IL-10的表达,计量观察DC与炎症因子在病程中的变化并做相关分析。结果伤后6h组,脾脏中CD11c+/MHC-Ⅱ+DC数目与IL-1β+细胞数目均明显增多,至24h组达峰值。自48h组,CD11c+/MHC-Ⅱ+DC与IL-1β+细胞含量开始下降,在6天组和10～12天组降至正常组或低于正常组含量;而同时CD11c+/MHC-Ⅱ-DC和IL-10阳性细胞含量则相对增多,至10～12天组阳性细胞含量显著升高达到峰值。结论DC的活性在MODS病程早期与IL-1β表达呈正相关性,在病程晚期与IL-10表达呈负相关,提示脾脏DC活性变化与促炎因子和抗炎因子均有密切的相关性。
Purpose To explore the effect of splenic dendritic cells （DCs） on the changes of inflammatoty cytokines in MODS mice. Methods Mice were randomized into two groups ： normal and MODS. The MODS mice were reproduced by zymosan injecting into the abdominal cavity. The immunophenotypes of spleenic DCs were detected by flow cytometry. Pro-inflammatory cytokine IL-1 β and antiinflammatory cytokine IL-10 in the spleen tissue were detected by immunohistochemical method. The relationship between splenic DC and IL-1β and IL-10 were analyzed. Results After zymosan trauma, the numbers of splenic CDllc＋/MHC-Ⅱ ＋ DCs and IL-1β cells obviously increased at hour 6, reached the peak at hour 24, but decreased from hour 48. On the 6th day and the 10 -12th days, the splenic CD11 c^＋/MHC- Ⅱ ＋ DCs numbers dropped to or under the normal level. At the same time, CD11 c ^＋/MHC- Ⅱ - DC and IL- 10 ^＋ cells increased relatively, and reached the maximum on the 10 - 12th days. Conclusions The activity of splenic DCs has positive relation with IL-1β at the early stage of MODS, while it has negative relation with IL-10 at the later stage of MODS. It suggests that splenic DCs have close correlation with the proinflammatory and anti-inflammatory cytokines in MODS.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2008年第6期716-720,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
全军医学科研"十一五"计划基金(06MB307)
首都医学发展科研基金(2003-3025)
