摘要
目的:建立兔角膜移植高危及非高危模型,通过阻断CD28,探讨CTLA4-Ig对高危角膜移植排斥反应的影响。方法:实验分为新生血管化模型组及非新生血管化组,每组随机分成:空白对照组(空白保存液)、实验组(浸入含有CTLA4-Ig10mg/L保存液4℃孵育18h),每组10只兔。观察术后受体植片角膜混浊情况和植片病理改变,原位杂交方法检测角膜植片TNF mRNA的表达情况,比较植片生存时间。结果:非新生血管化角膜移植组:对照组和实验各组的植片排斥时间或平均植片存活时间上无统计学意义,超过半数植片(16/30,53%)存活时间超过100d。新生血管化角膜移植组:实验组生存时间较长69±34d,对照组26±4d,原位杂交检测移植术后4wk或排斥反应发生时对照组角膜植片上皮下基质层浸润细胞有明显的TNF mRNA的表达,实验组未见TNF mRNA表达。结论:在兔角膜移植排斥反应中应用CTLA4-Ig阻断CD28,可以明显抑制兔高危角膜移植排斥反应,提高移植的存活率。
AIM. To study the effect of CTLA4-Ig on allograft rejection after high-risk corneal transplantation by blocking CD28 on the models of high-risk and non-high-risk rabbit corneal.
METHODS. Rabbits were divided into nonvascularized group and vascularized group, Then, each group was divided into control and experimental group seperately （n = 10）. Transplantant corneas were incubated in preserving solutions for control group, contained CTLA4-Ig （10mg/L） for experimental group （4℃, 18 hours）. All corneas were examined and the appearance of the graft was recorded with a slit-lamp photomicroscope after operation. Corneal grafts were examined on the 4^th week and rejection happened with histology method and in situ hybridization method. Tumor necrosis factor-α （TNF-α） on corneal graft was determined and survial days were compared between two parts.
RESULTS： Nonvascularized group, control allografts and all experimental allografts exhibited similar frequencies of rejection. More than half of the grafts （16/30, 53%） survived during the 100-day observation period; Vascularized group： the grafts of experimental group （69 ±34 days） had significantly longer mean survival times, compared with control group （26 ±4 days）, the expression of TNF-α was determined on corneal graft in control group in situ hybridization method, whereas no expression in experimental group.
CONCLUSION： These findings demonstrate that the blockade with CTLA4-Ig has a favorable effect to restrain the rejection of high-risk corneal transplantation.
出处
《国际眼科杂志》
CAS
2009年第2期271-273,共3页
International Eye Science
