摘要
目的:探讨肝癌辨证分型与肝癌细胞转移潜能的关系。方法:129例肝癌切除术后患者分为肝郁脾虚型、气血淤滞型、湿热蕴结型、肝肾阴虚型,采用免疫组化法检测不同辨证分型肝癌中E-cadherin/β-catenin蛋白质的差异,收集临床病理参数。结果:E-cadherin在癌灶中有细胞膜缺失和细胞质的异常表达,而且在不同证型中有差别。β-catenin在癌灶中异常表达较为明显。肝肾阴虚型患者肿瘤大小、数目、包膜、肝外转移和脾亢明显较肝郁脾虚严重,而门静脉癌栓无明显差别。四种辨证分型中肝功能Child分级和ICGR15变化最为明显,随辨证分型递增ICGR15其它三型与肝肾阴虚型之间有明显差别。湿热蕴结型HBV感染率明显高于其它三组。结论:原发性肝癌的辨证分型与E-cadherin/β-catenin细胞黏附有关,黏附紊乱依次为湿热蕴结型、气血瘀滞型、肝肾阴虚型、肝郁脾虚型,与肝功能相关,而且随着病程演进而变化。
Objective To explore the relationship between syndrome differentiation and metastasis potential of hepatoma careinoma cell. Methods One hundred and twenty - six cases of liver cancer were divided according to syn- drome differentiation into type of stagnation of liver - Qi with spleen deficiency, type of Retention of damp - heat, type of Qi- Blood stagnation, type of Liver- Kidney - Yin - Asthenia and Immunologic Type. Using inmmnohistochemical methods to detect the differences of the value of E - eadhefin protein and β - catenin protein, the clinicapathological parameter were collected. Results E - cadherin（E - CD） in the loci lost the cytoplasm of the cell, and there were some differences in different syndromes. β-catenin as much more markedly abnormal in loci. In patients with Liver- Kidney - Yin- Asthenia and hnmunologic Type, the situation of tumor and the spleen was obviously worse than that of those with syndrome of stagnation of liver - Qi with spleen deficiency, yet the situation of portal vein thronmbosis was not different. Conclusion Primary Liver Cancer Syndrome was related to the degree of adhesion of E - cadherin or β-catenin cells and liver function, associated with the course of evolution and change. In accordance with the disordered degree of cell adhesion, the first is the type of retention of damp - heat, then the type of Qi - blood stagnation, type of Liver - Kidney - Yin - Assthenia and Immunologic type, last is syndrome of stagnation of liver - Qi with spleen deficiency type.
出处
《中国中西医结合外科杂志》
CAS
2009年第1期10-12,共3页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
基金
广东省科技计划项目(2003C33906)
