摘要
目的:应用脉冲场凝胶电泳(PFGE)方法,对来自食物中毒标本中分离的沙门菌进行分子分型,探讨沙门菌食物中毒病原菌溯源方法和应用可能性。方法:菌株分离、生化鉴定和血清型确定参照GB4789.4-2003方法进行,对5株布利丹沙门菌用PFGE方法进行分子分型,应用Quantity oneTM分析软件对电泳后的条带进行分析。结果:从7份标本中检出5株布利丹沙门菌,检出率为71.4%,经PFGE分型,5株来源于食物中毒病人标本的布利丹沙门菌其带型一致,因此PFGE型别相同。结论:PFGE能用于沙门菌食物中毒细菌同源性的分析,在明确食物中毒诊断上有指导性作用,为沙门菌食物中毒溯源提供快速、可靠方法,值得推广、应用。
Objective ：To classify the Salmonella separated from poisoning food by the method of pulsed -field gel electropho- resis （PFGE） in molecular,then discuss the traceability of the pathogens of Salmonella from poisoning food and application possibilities. Methods： Strains isolated, biochemical identification and determining the serotype are done by referring to GB4789.4 - 2003 methods. Five S. blegdam are classified by PFGE method, then apply Quantity one TM electrophoresis analysis software to analyse of the band. Results：Five S. blegdam are found from the seven specimens and the detection rate was 71.4 %. The belt - line type of the five S. blegdam from poisoning is the same, so the type of the PFGE is similar. Conclusion：PFGE can be used to analyse the homology of the Salmonella from poisoning food ,play a guiding role in the clear diagnosis of poisoning food and provide a fast and reliable way for the Salmonella, thus, it is worth in the promotion and application.
出处
《中国卫生检验杂志》
CAS
2009年第1期116-117,158,共3页
Chinese Journal of Health Laboratory Technology
