摘要
目的探讨肿瘤坏死因子抗膀胱癌细胞作用的影响因素。方法将肿瘤坏死因子α(TNF-α)和不同浓度的丝裂霉素(MC)、顺铂(CDDP)以及干扰素α(IFN-α)作用于体外培养的膀胱癌细胞系BIU-87,用噻唑蓝(MTT)法测定细胞毒效果。结果 TNF-α对BIU-87细胞有明显的浓度依赖性抑制作用,MC(2.5～5.0mg/L)或IFN-α(2500U-5000U/ml)与TNF-α(10000U/ml)作用于细胞后的细胞活力及增殖明显低于单用TNF-α、IFN-α或MC实验组。结论 TNF-α与IFN-α或MC对膀胱癌细胞的抑制有协同作用。
Objective To investigate synergistic effects of tumor necrosis factor-a(TNF-α) with mytomyin C (MC), cisplatin (CDDP) or interferon-α(IFN-α) on human bladder cancer cells. Methods Human blad- der cancer cell line BIU-87 was cultured. The synergistic action of TNF-αwith MC. CDDP or IFN-αwas tested by MTT assay. Results The growth of BIU-87 cells was inhibited by TNF-α, in dose dependent manner. Both MM-TNF-αand IFN-α-TNF-αshowed synergistic action against the BIU-87 cell line. Con- clusions MC, INF-αand TNF-αare active agents against bladder cancer cell line.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1998年第1期83-84,共2页
Chinese Journal of Experimental Surgery
基金
甘肃省自然科学基金
关键词
肿瘤坏死因子Α
干扰素Α
膀胱肿瘤
药物疗法
Bladder cnacer
Tumor necrosis factor-α
lnterfcron-α
Mytornyin c
Cisplatin
