摘要
作者应用滤纸片干血液分离恶性疟原虫DNA进行聚合酶链反应的方法对所罗门群岛的489份血样品进行检查。结果表明此方法具有1）用血量少，经自然干燥后，易于保存和运输。2）DNA分离的方法简便、快捷。3）PCR的检测极限为1．5个原虫／μl血。其次，其结果与姬姆萨染色方法的结果进行了比较。结果提示姬姆萨染色方法的结果与其检查者的技术水平密切相关。在大面积普查时容易出现漏诊及误诊、此外，也证明单片段基因扩增同样容易出现漏诊及误诊。
To observe the value of application of polymerase chain reaction for the diagnosis of malaria in addition to lands． The results show：1）Filter paper disks can be used converiently for the collection of blood to detect malaria In PCR．and negatives are higher in rate than that of another method．3）The sensitivity was 72～79％ for the PCR-410bp and that was 62～79％ for the 618bp and specificity was 71～78％ and 83～87％，respectively．The sensitivity of PCR asimproved to 74～83％ by a combination of Solomon Islands and Japan．4）Among 50 positive amples proved by these two examiners，the sensitivity for 41obp was 88％ and that for618bp was 86％，the combination of both PCR was 92％．5）The detection limit for both PCR was nearly about 1．5 parasites/microlitre-blood（corresponding to 0．0000375％parasitemia level）.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1998年第1期33-36,共4页
Chinese Journal of Zoonoses
