摘要
[目的]研究茉莉酸甲酯对组培"Mona"百合鳞茎及鳞片叶生长的影响,以解决亚洲系统百合在试管鳞茎培养过程中鳞片生长过旺的问题。[方法]以亚洲型"Mona"百合试管鳞茎、鳞片的切片为外植体,研究茉莉酸甲酯对鳞片叶及鳞茎生长的影响。[结果]茉莉酸甲酯对"Mona"试管百合鳞片叶生长有抑制作用,浓度越高其效果越明显;当茉莉酸甲酯浓度为1.0μmol/L时,鳞片叶发生率(87.9%)明显低于对照(100.0%),但鳞茎分化和生长也显著好于对照;但茉莉酸甲酯浓度超过3.0μmol/L时,不仅对鳞片叶而且对鳞茎生长也有抑制效果。[结论]在"Mona"百合试管鳞茎培养的培养基中加入1.0μmol/L的茉莉酸甲酯时即可抑制鳞片叶生长也可促进鳞茎膨大生长。
[ Objective] The research aimed to study the effects of methy jasmonate （MeJA） on bulblet and scale leaf growth of Lilium ‘Mona＇ in vitro, to solve vigorous growth of scale leaves during tissue culture of bulblets in Asiatic Liliwn. [Method] Using bulblet and scale segments of Asiatic Lilium ‘Mona＇ in vitro, the effects of MeJA on the growth of scale leaves were investigated. [ Result] The scale leaf formation was inhibited by MeJA, the higher the concentration of MeJA the more efficient for restraining scale leaf growth. 100.0% of bulblet formation was obtained in the treatment of 1.0 μmol/L MeJA, but the scale leaf growth was significantly lower than control. 3.0μmol/L MeJA had the inhibitory effect to both the bulblets and the scale leaves. [ Conclusion] Application of MeJA is the efficient way for overcoming the problem of subculturing overelaborate during the tissue culture of Lilium ‘Mona＇, and 1.0 μmol/L MeJA is suitable for promoting bulblet growth and inhibiting scale leaf growth of Lilium ‘Mona＇.
出处
《安徽农业科学》
CAS
北大核心
2009年第1期39-39,82,共2页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金委资助项目(30560094)
国家教育部重点项目(205035)
