摘要
目的：研究人ＴＭＪ滑膜细胞（ＳＭＣ）蛋白质合成能力，与其它组织细胞的差异，及细胞因子对这方面的影响。方法：采用体外培养的ＳＭＣ、ＴＧＣ、ＮＭＣ３种细胞及无血清培养液，应用紫外分光光度仪在２８０ｎｍ波长处，对培养上清中的蛋白含量进行测定，并与加用ＩＬ－１、ＩＬ－６、ＴＮＦ后蛋白质合成的量进行对比观察。结果：正常状态下，ＴＧＣ蛋白合成量最高，ＯＤ值达０．１４，而ＳＭＣ最低，为０．０７９。在加用细胞因子作用４８ｈ后，ＳＭＣ蛋白含量达到０．１１７，提高３０％，而ＴＧＣ、ＮＭＣ却下降１０％～３０％。结论：正常状态下ＳＭＣ合成蛋白质成份较其它细胞少，而细胞因子可刺激ＳＭＣ蛋白质合成增加，这些蛋白成份中可能具有影响关节炎症的物质。
Objective:To investigate the protein synthesis of human TMJ synovial membrane cells (SMCs) in culture.Methods:SMCs,TGCs and NMCs were treated with IL 1 IL 6 and TNF. The protein contents in the supernatant of the cells were measured with spectrophotometer. Results:The obsorbance values for protein content of SMCs, TGCs and NMCs were 0.079, 0.14 and 0.1 respectively;That of SMCs increased by 30%,and that of TGCs and NMCs decreasedby 10%～30% after the cells had been treated with the cytokines. Conclusion:SMCs secrete less protein than TGCs and NMCs.Cytokines may stimulate SMCs to increase the secretion.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
1998年第2期139-140,共2页
Journal of Practical Stomatology
