摘要
本实验通过向密闭舱内灌注混合气体的方法，分别复制慢性缺氧、慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒大鼠模型。以地高辛标记的脑阴离子转运蛋白（ＡＥ３）ｃＤＮＡ探针，应用核酸原位杂交及点杂交技术，对ＡＥ３ｍＲＮＡ的分布及变化进行检测，结果显示：（１）ＡＥ３ｍＲＮＡ广泛分布于脑组织，其中以呼吸调整中枢所在部位脑桥深部灰质最为富集；（２）慢性缺氧组ＡＥ３ｍＲ－ＮＡ的相对含量显著地低于对照组（Ｐ＜０．０５），慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒组均非常显著地低于对照组（Ｐ＜０．０１）；（３）慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒组均非常显著地低于慢性缺氧组（Ｐ＜０．０１），但二组之间无显著性差异。提示ＡＥ３在呼吸衰竭的发生及其导致的肺性脑病中可能具有一定作用。
By pouring the mixed gases into the chamber,three rats models of hypoxia,hypoxia withhypercapnia and chronic acidosis were established respectively.With digoxigenin labelled cDNAprobe,in situ hybridization and dot blot were used to study the distribytion and the Change ofanion exchanger AE3 in rats brains in the three models.It was found that:(1)AE3 mRNA wasfound throughout the rat brain,the most abundant region was in pontine gray matter Which re-spected to be the control centre of respiratory(2)The relative contents of AE3 mRNA in ratbrains in experimental groups were significantly lower than that in control group(P<0·05),(3)the relative contents of AE3 mRNA in rat brain of hypoxia with hypercapnia and chronic aci-dosis were also significantly lower than that of chronic hypoxin (P<0.01),but there was nosignificant difference between chroic hypoxia with hypercapnia and chronic acidoas.These find-ings suggest that AE3 might play an important role in the respiratory failure and pulmonary en-cephalophathy。
出处
《基础医学与临床》
CSCD
1998年第2期51-55,共5页
Basic and Clinical Medicine
基金
国家自然科学基金
关键词
缺氧
二氧化碳潴留
脑
阴离子转运蛋白
肺性脑病
hypoxia hypercapnia anion exchanger pulmonary encephalophathy chloride channel
