摘要
运用流式细胞仪及细胞原位杂交方法测定低氧对培养的新生牛肺动脉平滑肌细胞（ＰＡＳＭＣ）生长的影响，并探讨血小板源生长因子（ＰＤＧＦ）在其中的作用。结果表明：培养的ＰＰＡＭＣ在低氧１２ｈ时，由Ｇ／Ｇ期进入Ｓ期的细胞数明显增多，为对照组的１５７．７％（Ｐ＜０．０５）：ＰＤＧＦ可进一步促进低氧状态下ＰＡＳＭＣ的增殖，Ｓ期细胞为对照组的２４２．３％（Ｐ＜０．０１）。原位杂交结果显示：低氧（１２ｈ）条件下，ＰＤＧＦ－Ａ．Ｂ链及ａ、ｂ受体基冈表达量显著增高（Ｐ＜０．００１）。提示：低氛条件下的ＰＤＧＦ及其受体基因表达增强与低氧的促增殖作用有协同效应，在低氧性肺血管重经过程中，可能是参与ＰＡＳＭＣ肥大、增生机制之一。
By means of flow cytometry and in situ hybridization ,we investigated the role of hypoxia in cultured neonatal bovine pu,lmonary artery smooth muscle cells (PASMC ) ,and the effect of PDGF on that role. Cell-cycle analysis revealed hypoxia ( 12h ) and PDGF added meantime had effect on the G/G S transition , both could increase the number of cells entering S phase from G/G The percents of S phase cells reached to 41%and 63%respectivly , and werel 57. 7% and 2.2. 3% compared with normoxiit (P<0. 05). Using in situ hybridization , we detected that hypoxia (12h ) upregulated PDGF-A,B chain and receptor gene expres- sion (all P<0. 001). Results therefore suggested that increase of PDGF-A ,B chain and receptor gene elxpression under hypoxia condition and effect of PDGF cooperated with hypox- ia on PASMCs proliferation may contribute to the pulmonary vascular remodeling.
出处
《基础医学与临床》
CSCD
1998年第2期33-37,共5页
Basic and Clinical Medicine
基金
国家自然科学基金
关键词
低氧
肺动脉
平滑肌细胞
PASMC增殖
PDGF
hypoxia pulmonary artery smooth muscle cell (PASMC) platelet-derived growth factor (PDGF)-A .B chain and receptor
