摘要
目的:探讨溃疡性结肠炎(ulcerative colitis,UC)大鼠结肠黏膜中性粒细胞趋化因子-1(CINC-1)及其受体CXCR2的表达及意义.方法:♂SD大鼠36只完全随机分为正常组、模型1组、模型2组,共3组,每组12只.用2,4,6三硝基苯璜酸100mg/kg灌肠建立大鼠溃疡性结肠炎模型,7d后处死模型1组动物,14d后处死模型2组动物.运用RT-PCR法、免疫组化染色法分别检测各组大鼠CINC-1及CXCR2mRNA及其蛋白的表达.结果:CXCR2主要表达在中性粒细胞及肠上皮细胞膜表面.UC活动期CINC-1及CXCR2mRNA及其蛋白表达明显呈上升趋势,与炎症相关.与正常组相比,模型1组、2组CINC-1mRNA及蛋白表达有显著差异(1.77±0.52,1.82±0.24vs0.29±0.10;0.30±0.01,0.31±0.04vs0.18±0.02,均P<0.05);CXCR2mRNA及蛋白表达也有显著差异(1.66±0.10,2.49±0.29vs0.55±0.13;0.20±0.03,0.23±0.02vs0.16±0.01,均P<0.05).结论:CINC-1及其受体CXCR2表达水平与UC炎症正相关,CINC-1可能通过CXCR2介导中性粒细胞浸润,造成结肠炎症及组织损伤.
AIM： To examine the expression and significance of cytokine-induced neutrophil chemoattractant-1 （CINC-1） and CXC chemokine receptor 2 （CXCR 2） in a rat model of colitis. METHODS： A total of 36 SD rats were randomly and equally divided into 3 groups with twelve rats in each group. A rat model of ulcerative colitis was established by an enema of 100 mg/ kg of TNBS and 500 mL/L ethanol, In model group 1, rats were sacrificed on day 7. The other rats were sacrificed on day 14. The expression of CINC-1 and CXCR2 were detected using the technique of immunohistochemical staining and RT- PCR. RESULTS： CXCR2 was predominantly expressed in the surface of neutrophil membrane and intestinal epithelial cell membrane. The mRNA and protein expression of CINC-1 and CXCR2 were on the increment in active UC. There was no association between UC and the expression of CINC-1 and CXCR2. Compared with normal group, there were significant differences between the mRNA and protein expression of CINC-1 in model group1 and model group 2 （1.77 ± 0.52, 1.82 ± 0.24 vs 0.29 ± 0.10; 0.30 ± 0.01, 0.31 ± 0.04 vs 0.18 ± 0.02, all P 〈 0.05）, also between the mRNA and protein expression of CXCR2 （1.66 ± 0.10, 2.49 ± 0.29 vs 0.55 ± 0.13; 0.20 ± 0.03, 0.23 ± 0.02 vs 0.16 ± 0.01, all P 〈 0.05）. CONCLUSION： There is a positive correlation between UC and the expression of CINC-1 and CXCR2. Our data suggest that CINC-1 may mediate the infiltration of polymorph nuclear neutrophil mainly through CXCR 2. Meanwhile, this change induces experimental colitis.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第1期78-82,共5页
World Chinese Journal of Digestology
基金
浙江省中医药管理局基金资助项目
No.2008CB060~~
