摘要
目的:探讨肥大细胞和降钙素基因相关肽(CGRP)对肠易激综合征(irritable bowel syndrome,IBS)发病机制的影响,为今后从神经-内分泌-免疫网络方面分析研究IBS的发生机制提供理论依据.方法:组织切片采用免疫组织化学的方法,用兔抗人降钙素基因相关肽多克隆抗体,测定染色阳性的CGRP,用特殊染色的方法测定染色阳性的肥大细胞.结果:四型IBS与对照组肠黏膜肥大细胞的数目相比在回盲部的分布不同(t=11.8,6.8,2.5,12.2,P<0.05或0.01);在乙状结肠部位只有腹泻型不同(t=2.1,0.01<P<0.05).IBS腹泻型患者回盲部、乙状结肠部肠黏膜中降钙素基因相关肽阳性神经肽纤维强度与对照组比较显著增强(11.13±1.3vs7.9±1.5,9±2.1vs7.5±1.2,P<0.05或0.01).IBS回盲部黏膜中肥大细胞数和降钙素基因相关肽阳性神经肽纤维强度的表达呈正相关(r=0.7,P<0.01).结论:肥大细胞和降钙素基因相关肽对IBS的发病可能有一定程度的影响.
AIM： To discuss whether mast cell （MC） and calcition gene related peptides （CGRP） have some influence on irritable bowel syndrome （IBS） and to offer theoretical basis for analysis of IBS from the perspective of nerve-endocrineimmune network. METHODS： Immunohistochemistry and rabbit anti-CGRP 1/2 polyclonal antibody were used to determine CGRP, and specific staining method was used to determine mast cells. RESULTS： The number of mast cells was greater in four types of IBS patients in the mucosa of ilececum compared with control group （t = 11.8, 6.8, 2.5, 12.2, P 〈 0.05 or 0.01）; in colon sigmoideum, only the diarrhea type had notable difference （t = 2.1, 0.01 〈 P 〈 0.05）. Patients with diarrhea type had significantly higher intensity of CGRP in ilececum and colon sigmoideum compared with control group （11.13 ± 1.3 vs 7.9 ± 1.5, 9 ± 2.1 vs 7.5 ± 1.2, P 〈 0.05 or 0.01）. The number of MC in mucosa of ilececum was positively correlated with expression power of CGRP （r = 0.7, P 〈 0.01）. CONCLUSION： MC and CGRP may have certain effect on the morbidity of IBS.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第2期213-217,共5页
World Chinese Journal of Digestology
关键词
肠易激综合征
肥大细胞
降钙素基因相关肽
Irritable bowel syndrome
Mast cell
Calcition gene related peptides
