摘要
目的:研究过度训练大鼠血浆和肾组织内皮素(ET-1)的变化及其意义,观察山莨菪碱的干预效应。方法:健康雄性Wistar大鼠56只随机分为对照组(CN,n=8)、力竭组(ES,n=32)和山莨菪碱组(AD,n=16)。对照组大鼠未进行力竭运动;力竭组又根据力竭后恢复时间分为力竭即刻(ESI)、力竭后6 h(ES 6 h)、力竭后12 h(ES 12 h)和力竭后24 h(ES24 h)组;山莨菪碱组于力竭运动前20 min腹腔注射山莨菪碱10 mg/kg后进行力竭运动,分为力竭后6 h(AD 6 h)及24 h(AD24 h)组。采用大鼠游泳至力竭建立过度训练模型;于力竭即刻、力竭后6 h、12 h和24 h分别检测各组大鼠血清尿素氮(BUN)、肌酐(Scr)的水平;光镜观察肾组织结构的改变;放射免疫分析法测定血浆和肾脏组织ET-1含量,采用Pearson法分析力竭大鼠血浆及肾组织ET-1与血清BUN和Scr的相关性。结果:力竭即刻大鼠血清BUN、Scr即明显升高(P<0.05),6 h达高峰(P<0.05),24 h恢复到对照水平。力竭后即刻、6 h、12 h及24 h大鼠肾组织结构变化轻微。力竭即刻组大鼠血浆和肾组织ET-1较对照组明显升高(P<0.01),力竭后6 h组升高更加明显(P<0.01),力竭后12 h组有所下降,但仍高于对照组(P<0.01),力竭后24 h组恢复达对照组水平。力竭大鼠血浆及肾组织ET-1含量与血清BUN和Scr均呈明显的正相关(P<0.001)。山莨菪碱组大鼠血浆和肾组织ET-1与同时间点力竭组比较明显降低(P<0.05),肾组织结构损伤减轻,肾功能明显改善(P<0.05)。结论:过度训练大鼠血浆和肾组织ET-1含量的变化主要参与力竭后早期肾损伤的发生,山莨菪碱可明显抑制过度训练引起的肾组织内皮素的过度生成,进而对过度训练大鼠起到明显的肾脏保护作用。
To study the role of endothelin （ET- 1 ） in development of overtraining- induced acute kidney injury （OTIAKI） and the effects of Anisodamine in exhausted rats. Methods：Fiftysix male Wistar rats were randomly divided into three groups, control group （CN, n = 8）, exhaustively swimming （ES, n = 32 ）, Anisodamine （AD, n = 16）. The rats of CN were quiet and not swum. The rats of ES were swum to exhaustion and sacrificed immediately （ESI）,6 hour （ES 6 h）, 12 hour （ES 12 h） and 24 hour （ES 24 h） after exhaustively swimming. The rats of AID received intraperitoneal injection of Anisodamine at the dose of 10 mg/kg body weight at 20 minutes before swimming and then were swum to exhaustion. The animal model of overtraining-induced acute kidney injury was established by exhaustively swimming. The contents of plasma and renal tissue ET- 1 were measured by the way of radio- immunoanalyzing. The interrelation between the content of ET - 1 and the value of serum BUN and Scr was analysed by Pearson method. Results：The levels of serum BUN and Scr increased markedly in ESI rats （P 〈 0.05）, higher in ES 6 h rats （P （0.05）, and returned to CN levels in ES 24 h rats. HE staining showed that the morphological changes were slight in ES rats. Radio-immunoanalyzing showed that the levels in plasma and renal tissue ET- 1 increased significantly in ESI rats and increased to top levels in ES 6h rats （P （0.01）, and decreased gradually in ES 12 h to ES 24 h, and returned to CN levels in ES 24h rats. The content of plasma and renal tissue ET- 1 had significantly positive correlation with the value of serum BUN and Scr （P〈0.01）. Conclusion：These results suggest that the changes of plasma and renal tissue ET- 1 levels could mainly take part in the development of early stage kidney injury in OTIAKI;the pre- treatment with Anisodamine exerts renoprotective effects on overtraining -induced acute kidney iniury,prohahly through the suppression of renal ET- 1 overproduction.
出处
《中国中西医结合肾病杂志》
2009年第2期120-123,I0002,共5页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
全军"十一五"医药卫生科研基金资助项目(No.06MA071)
