摘要
目的:研究nestin(巢蛋白)在人尿萃取物细胞分化剂CDA-2(又名尿多酸肽)诱导SWO-38人胶质瘤细胞分化中表达的变化及其意义。方法:采用光镜观察和鉴定CDA-2对SWO-38细胞的分化作用;RT-PCR、细胞免疫荧光、Western blotting分析CDA-2诱导前后SWO-38细胞nestin表达的变化。结果:光镜观察发现经CDA-2诱导后SWO-38细胞胞体变大、核浆比减小、突起增多,表现出向成熟的星形细胞分化的现象;Nestin表达在细胞浆,呈丝状着色;Nestin在CDA-2诱导SWO-38细胞分化中表达下调。结论:Nestin在CDA-2诱导SWO-38细胞分化中表达下调,进一步验证nestin与细胞分化的关系,nestin有可能成为胶质瘤诱导分化治疗领域新的标志物。
AIM： To investigate the change and significance of nestin expression in differentiation of human glioma cell line SWO -38 induced by CDA -2 （uroacitide, a healthy human urine extract）. METHODS： Cellular differentiation of SWO - 38 cells induced by CDA - 2 was determined by light microscopy. The change of nestin expression in SWO- 38 cells induced by CDA- 2 was detected by immunofluorescence, RT- PCR and Western blotting. RESULTS： Light microscopic observation revealed that CDA- 2 induced SWO -38 cells to differentiate into astrocytes with increased cytoplasm and cytoplasmic processes and decreased nucleus/cytoplasm ratio. Nestin was expressed in cytoplasm and stained like filament by immunofluorescence staining. Nestin expression was downregulated in differentiated SWO - 38 cells induced by CDA-2. CONCLUSION： Nestin expression is downregulated in differentiation of SWO-38 cells induced by CDA - 2, which verifies the relationship between nestin expression and cell differentiation. Nestin may be a new differentiation marker of glioma.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第2期226-230,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30670795)
广东省自然科学基金资助项目(No.06025208)
高等学校博士学科点专项科研基金资助项目(No.20040559008)
关键词
细胞分化剂
神经胶质瘤
巢蛋白
细胞分化
Cell differentiation agent
Glioma
Nestin
Cell differentiation
