摘要
目的：探讨胶体金法和ELISA法在儿童HBsAg检测中的作用，了解某县1～7岁儿童HBsAg感染趋势。方法：对2004～2008年某县幼儿园69 303名儿童采用胶体金法筛查乙肝表面抗原，初筛阳性者再用ELISA法复查两对半。结果：经胶体金法筛查乙肝表面抗原阳性者947例，ELISA法复查两对半确诊为乙肝者878例；2004—2008年阳性率分别为1．77％，1．34％，1．31％，1．23％，0．87％，各年度HBsAg阳性率比较差异有显著性（P〈0．05）。结论：某县儿童乙肝表面抗原阳性率呈逐年下降趋势；胶体金法作为儿童HBsAg初筛仍是一种理想的方法。
Objective ：To discuse the role of immunogold staining method and ELISA in examming HBsAg of children. To observe the HBsAg positive rate and tendency of the children in one county. Methods：There were 69 303 cases, from 2004 to 2008 ,first screening HBsAg with the immunogold staining method, then the detection of HBsAg markers of the postive cases was made with ELISA. Results ：The cases of immunogold staining method were 947. The cases of ELISA were 878. The postive rate of HBsAg from 2004 to 2008 was 1.77%, 1.34%, 1.31%, 1.23% ,0. 87% respectively. There were significant differences （ P 〈 0.05 ）. Conclusion ： The HBsAg positive rate of the children in our eontry shows a downward trend, immunogold staining method is still an ideal method as a child surface antigen screening.
出处
《现代临床医学》
2009年第1期43-45,共3页
Journal of Modern Clinical Medicine
