摘要
本文将３０头杂交山羊分成３组，第１组（ｎ＝６）为对照组，第２组（ｎ＝１２）静注大肠杆菌内毒素复制休克模型，第３组（ｎ＝１２）在第２组基础上提前１０ｍｉｎ静注山莨菪碱注射液，检测１２ｈ内不同时间血清丙二醛（ＭＤＡ）含量、总ＳＯＤ、Ｍｎ－ＳＯＤ、ＣｕＺｎ－ＳＯＤ、ＣＡＴ和血液ＧＳＨ－Ｐｘ活性。结果表明：第２组血清ＭＤＡ含量明显增加（Ｐ＜０．０１），血清Ｔ－ＳＯＤ、Ｍｎ－ＳＯＤ、ＣｕＺｎ－ＳＯＤ、ＧＳＨ－Ｐｘ、ＣＡＴ活性均显著降低（Ｐ＜０．０１），而第３组血清ＭＤＡ含量虽比第１组增加，至７ｈ后则无显著差异，并与第２组差异明显（Ｐ＜０．０１），各类抗氧化酶的活性比第２组下降幅值明显减小。提示脂质过氧化损伤在内毒素休克发生发展中起一定作用，应用６５４－２可降低脂质过氧化作用而有效地保护细胞功能和结构正常。
hirty hybrid goats were randomly divided into three groupsGroup Ⅰ (n=6) was the normal controlThe goats of group Ⅱ (n=12) were intravenously injected with Ecoli endotoxin as an endotoxin shock modelThe goats of group Ⅲ (n=12) were intravenously injected with anisodamine 10 minute before endotoxin injectionThe serum MDA contents,total SOD,CuZnSOD,MnSOD,catalase and GSHPx activities were examined at different timesThe results showed that serum MDA contents of group Ⅱ significantly (P<001) increased and activities of serum total SOD,MnSOD,CuZnSOD,catalase and blood GSHPx markedly (P<001) decreased after endotoxin injectionSerum MDA contents of group Ⅲ increased in comparison with group Ⅰ,however,there was no significant difference after seven hoursThe activities of antioxidant enzymes of group Ⅲ lowered less than those of group ⅡThe experimental results indicated that lipid perioxidation played a certain role in tissue injury of endotoxic shock of goatsThe anisodamine could reduce the lipid peroxidation and effectively protect cell functions and structure
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1998年第2期179-184,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金
