摘要
目的:建立款冬花药材中芦丁的含量测定方法并比较不同来源的款冬花中芦丁的含量。方法:采用HLPC法。色谱柱:迪马公司Diamonsil C18(150mm×4.6mm,5μm);流动相:甲醇-0.1%磷酸溶液(38:62);流速:1.0mL.min-1;检测波长:354nm;温度:30℃。结果:芦丁进样量在0.09～0.98μg范围内线性关系良好(r=0.9999),平均回收率(n=9)为100.6%,不同来源药材中芦丁的含量范围为0.19%～0.39%。结论:不同产地的款冬花药材中芦丁含量差异不大。
Objective：To develop an HPLC method to determine the contents of rutin in Tussilago farfara from different areas. Method： Diamonsil C18 column（ 150 mm × 4.6 mm,5 μm） was adopted;the mobile phase consisted of methanol -0. 1% phosphoric acid solution（38： 62） at a flow rate of 1.0 mL · min^-1 ;the detection wavelength was 354 nm, and the temperature was 30 ℃. Results：The calibration curve was linear in the range of 0.09 - 0.98 μg（ r = 0. 9999 ） ; The average recovery（ n = 9 ） was 100.6%. The contents of rutin in Tussilago farfara from different areas were 0. 19% -0. 39%. Conclusion： Tussilago farfara of different areas contains similar amount of rutin.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第1期134-136,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
HPLC
款冬花
芦丁
HPLC
Tussilago farfara L.
rutin
