摘要
背景:低强度激光生物效应已经越来越受到科学工作者和临床医生的重视,其在不引起机体产生不可逆性损伤的前提下对机体代谢的积极调节作用让人们对于它临床上的投入前景非常期待。目的:观察630nm和808nm低强度激光单独照射和联合照射对大鼠背部皮肤创伤愈合的影响。设计、时间及地点:随机对照动物实验,于2008-05/07在天津医科大学完成。材料:清洁级雄性Wistar大鼠32只,体质量(240±20)g。方法:用手术剪在每只Wistar大鼠背部左右对称各造2个圆形皮肤全层切割型创口,4个创口按部位统一编号。将32只大鼠按完全随机分组法分为4组,空白对照组、630nm单波长低强度激光照射组、808nm单波长低强度激光照射组和630/808nm双波长低强度激光照射组,每组8只。3个照射组中的大鼠背部创口分别用不同波长低强度激光连续照射14d,1次/d,每个创口照射10min/次;空白对照组无任何干预措施。主要观察指标:伤后每隔1d用透明薄膜描记每只大鼠Ⅰ号创口的创面,根据积分法计算创面面积收缩率;伤后第3,7,14天对创口取活检做常规苏木精-伊红染色,光镜观察组织形态变化。结果:32只Wistar大鼠均进入结果分析。①与空白对照组相比,630/808nm双波长低强度激光照射组和630nm单波长低强度激光照射组创伤面积收缩速度快,差异具有显著性意义(P<0.05)。②630/808nm双波长低强度激光照射组和630nm单波长低强度激光照射组较其他2组肉芽组织出现早,同时成纤维细胞的数量与胶原分泌量多、创伤愈合情况好。结论:630nm单波长或630/808nm双波长低强度激光照射均能有效促进大鼠皮肤创伤的愈合过程。
BACKGROUND： Effects of low-intensity laser irradiation （LILI） within the red and near-infrared spectrum on wound healings have become a hot topic. It plays a positive role in body metabolism and not produces irreversible damage. OBJECTIVE： To evaluate effects of LILI at 630 or/and 808 nm on cutaneous wound healings of Wistar rats. DESIGN, TIME AND SETTING： Randomized controlled animal experiment was performed at the Tianjin Medical University from May to July 2008. MATERIALS： Thirty-two clean male Wistar rats weighing （240±20） g were selected. METHODS： Four circular excisions were made on the dorsum of each rat, and numbered. The rats were randomly divided into 4 groups： control, LILI at 630 nm, LILI at 808 nm and LILI at 630 and 808 nm groups （n--8）. The wound of three treated groups were irradiated 10 minutes each, once a day for a consecutive 14 days. The control group did not receive any treatment. MAIN OUTCOME MEASURES： The outline of one of the four wounds in each rat was recorded every other day and their areas were calculated. The other three wounds in each rat were biopsied separately and specimens were observed after HE staining under microscope. RESULTS： All 32 rats were included in result analysis. The area reductions in LILI at 630 and 808 nm and LILI at 630 nm groups were significantly rapid than control group （P 〈 0.05）. Granulation tissues arose earlier in LILI at 630 and 808 nm and LILI at 630 nm groups than the other groups, In addition, the number of fibroblast and quantity of collagen production was more and the wound healing was better in LILI at 630/808 nm and at 630 nm groups than the other groups. CONCLUSION： LILI at 630 nm alone or in combination of 630 and 808 nm could significantly improve cutaneous wound healings.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第52期10254-10258,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
