摘要
目的:探讨外毛细胞维持形态及变性过程发生的可能机制。方法:倒置相差显微镜下,对于急性酶分离所得的外毛细胞进行6 h的连续性观察。结果:半数以上活性良好的单离外毛细胞可保持活性平均约6 h,变性过程中均出现自表皮板至基底部的纵行褶线。含钙和不含钙的细胞维持活性时间相同。静纤毛缺失可以同样维持较长时间的活性。结论:钙离子浓度和静纤毛损伤不是外毛细胞变性的决定性因素,而环细胞表面的某种弹性机制可能是维持形态保持活性的根本原因。
Objective： To investigate the degeneration mechanics of outer hair cells in guinea pig. Method： The mechanics of outer hair cells isolated by enzyme were observed under inverted microscope for 6-8 h continuously. Result：Over half of living outer hair cells could keep good conditions in 6 hours. During the degeneration there was always a longitudinal fold line from tip to base. Presence or absence of calcium, as well as lossing of stereociliary bundle, couldn＇t change the conditions of out hair cells. Conclusion： Neither calcium nor stereociliary bundle is the decisive cause in keeping outer hair cells alive, and its degeneration may be basically related with something surrounding the cell.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2008年第23期1087-1089,共3页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
外毛细胞
变性
形态学
Outer hair cell
Degeneration
Morphology
