摘要
背景:研究发现,脐血间质干细胞对辐射损伤有一定的防护作用。目的:实验拟观察脐血间质干细胞静脉输注对照射小鼠脾脏细胞生物学效应的影响,验证其防辐射损伤作用。设计、时间及地点:随机对照动物实验,于2007-04/10在广东医学院实验动物中心完成。材料:将体质量(20.0±1.5)g的雌性昆明种小鼠随机分为正常对照组、单纯照射对照组、60Coγ射线照射前静脉注射脐血间质干细胞组,每组30只。新鲜脐带血来源于本院妇产科健康产妇。方法:以无菌塑料采血袋按密闭式方法采集足月新生儿脐带血80～140mL,无菌条件下将脐带血液采集到含有ACD-B保养液的无菌采血袋内,并按照卫生部检测血液的标准检测每一份脐带血液,采集、分离间质干细胞。60Coγ射线照射小鼠,通过尾静脉将分离获得的人脐血间质干细胞输注入小鼠体内,细胞数≥1×108/份,照射后当天再次输注人脐血间质干细胞。主要观察指标:应用流式细胞仪检测脾脏细胞的凋亡指数和免疫参数。结果:与单纯照射组相比,60Coγ射线照射前静脉注射脐血间质干细胞组的G0/G1期细胞比例下降,而S、G2M期细胞比例均有不同程度的升高。经照射后,小鼠脾细胞的凋亡率明显增加,脐血间质干细胞对细胞的凋亡有一定保护作用,同时能够提高细胞的增殖率。单纯照射组的NK细胞、CD4+和CD8+标记率明显低于正常对照组,而脐血间质干细胞能显著地提高它们的活性。结论:脐血间质干细胞静脉输注对辐射造成的脾细胞损伤有一定保护作用,可促进脾脏细胞的分裂增殖,加速脾脏细胞的修复,其作用机制可能与提高NK细胞活性和CD4+、CD8+数量有关。
BACKGROUND： Umbilical cord blood mesenchymal stem cells （UCB-MSCs） have safety action on radiation damage. OBJECTIVE： To study the effects of vein transplantation of human UCB-MSCs on cell biology activity of spleen lymphocyte subsets in irradiated mice, and to verify effects on anti-radiation damage. DESIGN, TIME AND SETTING：,The randomized control animal experiment was performed at the Animal Laboratory Center of Guangdong Medical College from April to October 2007. MATERIALS： The female Kunming mice weighing （20±1.5） g were randomly divided into a normal control group, simple radiated group, ^60Co γ radiation ＋ UCB-MSCs group, 30 mice in each group. Fresh umbilical cord blood was collected from healthy parturients from Department of Gynaecology and Obstetrics of this hospital. METHODS： Full-term neonatal UCB （80-140 mL） was collected using closed type method with sterile plastics containing ACD-B conserve medium. UCB was detected according to criteria of Health Ministry. MSCs were isolated. Mice were irradiated using ^60Co y ray. Human UCB-MSCs were infused into mice via the tail vein, ≥ 1×10^8 every sample. Human UCB-MSCs were infused again immediately after irradiation. MAIN OUTCOME MEASURES： Apoptotic index and immune reference of spleen lymphocytes using flow cytometry. RESULTS： Compared with the simple radiated group, cell proportion decreased in the G0/G1 phase in the ^60Co γ radiation ＋ UCB-MSCs group, but increased in the S and G2M phases to some degrees. After irradiation, apoptotic rate of mouse splenocytes significantly increased. UCB-MSCs had protective effects on cell apoptosis, and also elevated cell proliferative rate. Natural killer cells, CD4^＋ and CD8^＋ labeling rates were significantly lower in the simple radiated group compared with the normal control group, and UCB-MSCs could significantly increase their activity. CONCLUSION： Vein transplantation of human UCB-MSCs has protective effects on splenocyte damages induced by irradiation, can promote prol
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第1期145-148,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
