摘要
目的:研究高同型半胱氨酸血症(HHcy)致大鼠动脉粥样硬化的作用机制。方法:20只大鼠平均分为对照组和HHcy组。对照组给予分析化学家协会(AOAC)推荐的配方饲料,HHcy组则给予在AOAC配方基础上添加3%L-蛋氨酸的饲料。用高效液相色谱法测定血清同型半胱氨酸(Hcy)含量,分析血清一氧化氮(NO)、一氧化氮合酶(NOS)、内皮素-1(ET-1),丙二醛(MDA)、羟自由基(OH-)、过氧化氢(H2O2)、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和乳酸脱氢酶(LDH)等。取大鼠腹主动脉作病理学检查和电镜观察。结果:HHcy组大鼠血清Hcy含量均随时间的延长而显著提高,NO含量显著低于对照组,而ET-1含量显著高于对照组(P<0.01),两组间NOS含量无明显差异。病理学检查显示,对照组主动脉内膜完整,无脂肪颗粒沉着;HHcy组主动脉内膜中有泡沫细胞聚集,有脂肪颗粒沉着。HHcy组MDA和H2O2含量显著高于对照组(P<0.01)。OH-含量也明显高于对照组(P<0.05),而T-AOC含量较对照组显著降低(P<0.01)。HHcy组SOD和GSH-Px活性较对照组显著降低(P<0.01),LDH较对照组显著提高。两组间CAT无显著差异。结论:Hcy可通过氧化应激机制损伤血管内皮细胞,促进动脉粥样硬化的形成。
Objective： To investigate the mechanism of hyperhomocysteninemia （HHcy） which induces atherogenesis in rats. Methods： 20 male rats were randomly divided into control group fed with AOAC diet and HHcy group fed with AOAC diet plus 3% L-methionine for 12 weeks. Serum levels of Hcy,NO, NOS, ET-1, MDA, OH -, H2 02 ,T-AOC, SOD, GSH-Px, CAT, LDH were determined, and the pathology of abdominal aortas were analyzed. Results： Moderate hyperhomocysteinemia were induced in HHcy group rats. Serum levels of NO were lower and ET-1 were higher in HHcy group rats than in control group rats （P 〈0.01 ）. There was no difference of serum levels of NOS in two groups. There were some foam cells and deposition of lipochondria in aortic tunica intima in HHcy group rats, which were not found in control group rats. Serum levels of MDA, H202, OH- were higher,and T-AOC were lower in HHcy group rats than in control group rats （P 〈 0.01 ）. Serum activities of SOD and GSH-Px were lower and LDH were higher in HHcy group rats than in control group rats（ P 〈 0. 01 ）. There was no difference of serum activities of CAT in two groups. Conclusion ： HHcy may injure vascular endothelium and promote atherogenesis by oxidative stress.
出处
《肠外与肠内营养》
CAS
北大核心
2009年第1期7-11,共5页
Parenteral & Enteral Nutrition
